Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1985 Jul;78(3):484–488. doi: 10.1104/pp.78.3.484

Effect of Low Temperature and Calcium on Survival and Membrane Properties of Isolated Winter Wheat Cells 1

M Keith Pomeroy 1, Chris J Andrews 1
PMCID: PMC1064763  PMID: 16664270

Abstract

Isolated cells obtained by enzymic digestion of young primary leaves of cold-hardened, dark-grown Kharkov winter wheat (Triticum aestivum L.) were exposed to various low temperature stresses. The initial uptake of 86Rb was generally decreased by increasing concentrations of Ca2+, but after longer periods of incubation, the inhibiting effect of high Ca2+ levels diminished. Viability of isolated cells suspended in water declined rapidly when ice encased at −1°C, while in the presence of 10 millimolar Ca2+ viability declined only gradually over a 5-week period. Ice encasement markedly reduced 86Rb uptake prior to a significant decline in cell viability or increased ion efflux. Cell damage increased progressively when the icing temperature was reduced from −1 to −2 and −3°C, but the presence of Ca2+ in the suspending medium reduced injury. Cell viability and ion uptake were reduced to a greater extent following slow cooling than after rapid cooling to subfreezing temperatures ranging from −10 to −30°C. The results from this study support the view that an early change in cellular properties due to prolonged ice encasement at −1°C involves the ion transport system, whereas cooling to lower subfreezing temperatures for only a few hours results in more general membrane damage, including loss of semipermeability of the plasma membrane.

Full text

PDF
485

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews C. J., Pomeroy M. K. Toxicity of Anaerobic Metabolites Accumulating in Winter Wheat Seedlings during Ice Encasement. Plant Physiol. 1979 Jul;64(1):120–125. doi: 10.1104/pp.64.1.120. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Goodenough-Trepagnier C., Tarry E., Prather P. Derivation of an efficient nonvocal communication system. Hum Factors. 1982 Apr;24(2):163–172. doi: 10.1177/001872088202400202. [DOI] [PubMed] [Google Scholar]
  3. Harrington H. M., Berry S. L., Henke R. R. Amino Acid Transport into Cultured Tobacco Cells: II. EFFECT OF CALCIUM. Plant Physiol. 1981 Feb;67(2):379–384. doi: 10.1104/pp.67.2.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Mettler I. J., Leonard R. T. Ion transport in isolated protoplasts from tobacco suspension cells: I. General characteristics. Plant Physiol. 1979 Jan;63(1):183–190. doi: 10.1104/pp.63.1.183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Pomeroy M. K., Andrews C. J. Metabolic and ultrastructural changes associated with flooding at low temperature in winter wheat and barley. Plant Physiol. 1979 Oct;64(4):635–639. doi: 10.1104/pp.64.4.635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Pomeroy M. K., Pihakaski S. J., Andrews C. J. Membrane properties of isolated winter wheat cells in relation to icing stress. Plant Physiol. 1983 Jun;72(2):535–539. doi: 10.1104/pp.72.2.535. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Poovaiah B. W., Leopold A. C. Effects of inorganic salts on tissue permeability. Plant Physiol. 1976 Aug;58(2):182–185. doi: 10.1104/pp.58.2.182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Rains D. W., Floyd R. A. Influence of calcium on sodium and potassium absorption by fresh and aged bean stem slices. Plant Physiol. 1970 Jul;46(1):93–98. doi: 10.1104/pp.46.1.93. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Singh J. Isolation and Freezing Tolerances of Mesophyll Cells from Cold-hardened and Nonhardened Winter Rye. Plant Physiol. 1981 May;67(5):906–909. doi: 10.1104/pp.67.5.906. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Smith I. K. Role of calcium in serine transport into tobacco cells. Plant Physiol. 1978 Dec;62(6):941–948. doi: 10.1104/pp.62.6.941. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES