Abstract
The effects of sulfur deficiency on the complement of proteins laid down in developing seeds of soybean (Glycine max L. Merr) have been examined. Sulfur deficiency caused a 40% decrease in the level of glycinins and a contrasting elevation in the level of β-conglycinins. The subunit composition of these proteins was also affected. There was in particular a 3-fold increase in the β-subunit of β-conglycinins in the sulfur-deficient seeds, and this accumulated largely as the B0-isomer of β-conglycinins, a protein which while virtually devoid of methionine and cysteine retains the physical properties of a normal 7S storage protein. These data demonstrate that a high degree of selectivity can be exerted by environmental stress over the accumulation of proteins in developing seeds.
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- Chandler P. M., Higgins T. J., Randall P. J., Spencer D. Regulation of Legumin Levels in Developing Pea Seeds under Conditions of Sulfur Deficiency: Rates of Legumin Synthesis and Levels of Legumin mRNA. Plant Physiol. 1983 Jan;71(1):47–54. doi: 10.1104/pp.71.1.47. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gayler K. R., Sykes G. E. beta-Conglycinins in Developing Soybean Seeds. Plant Physiol. 1981 May;67(5):958–961. doi: 10.1104/pp.67.5.958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holowach L. P., Thompson J. F., Madison J. T. Storage Protein Composition of Soybean Cotyledons Grown In Vitro in Media of Various Sulfate Concentrations in the Presence and Absence of Exogenous l-Methionine. Plant Physiol. 1984 Mar;74(3):584–589. doi: 10.1104/pp.74.3.584. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Schuler M. A., Ladin B. F., Pollaco J. C., Freyer G., Beachy R. N. Structural sequences are conserved in the genes coding for the alpha, alpha' and beta-subunits of the soybean 7S seed storage protein. Nucleic Acids Res. 1982 Dec 20;10(24):8245–8261. doi: 10.1093/nar/10.24.8245. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schuler M. A., Schmitt E. S., Beachy R. N. Closely related families of genes code for the alpha and alpha' subunits of the soybean 7S storage protein complex. Nucleic Acids Res. 1982 Dec 20;10(24):8225–8244. doi: 10.1093/nar/10.24.8225. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spencer D., Higgins T. J., Button S. C., Davey R. A. Pulse-labeling Studies on Protein Synthesis in Developing Pea Seeds and Evidence of a Precursor Form of Legumin Small Subunit. Plant Physiol. 1980 Sep;66(3):510–515. doi: 10.1104/pp.66.3.510. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Staswick P. E., Hermodson M. A., Nielsen N. C. Identification of the acidic and basic subunit complexes of glycinin. J Biol Chem. 1981 Aug 25;256(16):8752–8755. [PubMed] [Google Scholar]
- Sykes G. E., Gayler K. R. Detection and characterization of a new beta-conglycinin from soybean seeds. Arch Biochem Biophys. 1981 Sep;210(2):525–530. doi: 10.1016/0003-9861(81)90217-4. [DOI] [PubMed] [Google Scholar]
- Thanh V. H., Shibasaki K. Beta-conglycinin from soybean proteins. Isolation and immunological and physicochemical properties of the monomeric forms. Biochim Biophys Acta. 1977 Feb 22;490(2):370–384. doi: 10.1016/0005-2795(77)90012-5. [DOI] [PubMed] [Google Scholar]