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. 1985 Aug;78(4):812–816. doi: 10.1104/pp.78.4.812

Homology among 3S and 7S Globulins from Cereals and Pea

Laurian S Robert 1, Khosrow Adeli 1, Illimar Altosaar 1
PMCID: PMC1064828  PMID: 16664331

Abstract

The globulins from wheat caryopses were found to consist primarily of protein sedimenting at approximately 3S and 7S. These proteins displayed a molecular weight distribution similar to that of the purified vicilin-like fractions from oat and pea, with variations occurring in the isoelectric points and relative quantities of their major subunits. concanavalin A Sepharose chromatography suggested that the major polypeptides of the wheat (3S + 7S) fraction are glycosylated. Western blot analysis using antioat (3S + 7S) globulin immunoglobulin G revealed the vicilins from pea and the globulin fractions of oat, wheat, barley, rye, corn, and rice to contain immunologically homologous polypeptides. Major groups of polypeptides were shared by all the cereals and pea, including subunits of approximately 75, 50, 40 kilodaltons and 20 to 25 kilodaltons. These results indicate that legume-like 3S and 7S globulins have been conserved and are being expressed in cereals.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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