Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1982 Sep;70(3):827–832. doi: 10.1104/pp.70.3.827

Glutamate Synthase Isoforms in Rice

Immunological Studies of Enzymes in Green Leaf, Etiolated Leaf, and Root Tissues

Akira Suzuki 1,1, Jean Vidal 1, Pierre Gadal 1
PMCID: PMC1065778  PMID: 16662583

Abstract

Rabbit antiserum was raised against ferredoxin-dependent glutamate synthase (EC 1.4.7.1) purified from green leaves of Oryza sativa L. cv Delta. Ferredoxin-dependent glutamate synthase, detected in green leaf, etiolated leaf, and root tissues cross-reacted completely with the antiferredoxin glutamate synthase immunoglobulin G. In contrast, the immunoglobulin G did not cross-react with NADH-dependent (EC 1.4.1.14) and NADPH-dependent (EC 1.4.1.13) glutamate synthases found in nonphotosynthetic etiolated leaf and root tissues. In addition, ferredoxin-dependent glutamate synthase was separated and distinguished by its affinity to ferredoxin from NAD(P)H-dependent glutamate synthase on ferredoxin-Sepharose affinity chromatography. Based on the immunological studies, it is suggested that ferredoxin-dependent glutamate synthases in green leaf and etiolated leaf tissues are closely related proteins; in contrast, ferredoxin-dependent glutamate synthase in root tissue is a distinct protein from the leaf enzymes.

Full text

PDF
830

Images in this article

828Fig. 1
on p.828
829Fig. 3
on p.829
831Fig. 5
on p.831

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beevers L., Storey R. Glutamate Synthetase in Developing Cotyledons of Pisum sativum. Plant Physiol. 1976 Jun;57(6):862–866. doi: 10.1104/pp.57.6.862. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boland M. J., Benny A. G. Enzymes of nitrogen metabolism in legume nodules. Purification and properties of NADH-dependent glutamate synthase from lupin nodules. Eur J Biochem. 1977 Oct 3;79(2):355–362. doi: 10.1111/j.1432-1033.1977.tb11816.x. [DOI] [PubMed] [Google Scholar]
  3. Fowler M. W., Jessup W., Sarkissian G. S. Glutamate synthetase type activity in higher plants. FEBS Lett. 1974 Sep 15;46(1):340–342. doi: 10.1016/0014-5793(74)80401-1. [DOI] [PubMed] [Google Scholar]
  4. Haslett B. G., Cammack R., Whatley F. R. Quantitative studies on ferredoxin in greening bean leaves. Biochem J. 1973 Nov;136(3):697–703. doi: 10.1042/bj1360697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hirel B., Perrot-Rechenmann C., Suzuki A., Vidal J., Gadal P. Glutamine Synthetase in Spinach Leaves : IMMUNOLOGICAL STUDIES AND IMMUNOCYTOCHEMICAL LOCALIZATION. Plant Physiol. 1982 Apr;69(4):983–987. doi: 10.1104/pp.69.4.983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lea P. J., Miflin B. J. Alternative route for nitrogen assimilation in higher plants. Nature. 1974 Oct 18;251(5476):614–616. doi: 10.1038/251614a0. [DOI] [PubMed] [Google Scholar]
  7. Lea P. J., Miflin B. J. The occurrence of glutamate synthase in algae. Biochem Biophys Res Commun. 1975 Jan 2;64(3):856–862. doi: 10.1016/0006-291x(75)90126-6. [DOI] [PubMed] [Google Scholar]
  8. McKenzie G. H., Ch'ng A. L., Gayler K. R. Glutamine Synthetase/Glutamine: alpha-Ketoglutarate Aminotransferase in Chloroplasts from the Marine Alga Caulerpa simpliciuscula. Plant Physiol. 1979 Mar;63(3):578–582. doi: 10.1104/pp.63.3.578. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Miflin B. J., Lea P. J. Glutamine and asparagine as nitrogen donors for reductant-dependent glutamate synthesis in pea roots. Biochem J. 1975 Aug;149(2):403–409. doi: 10.1042/bj1490403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Miller R. E., Stadtman E. R. Glutamate synthase from Escherichia coli. An iron-sulfide flavoprotein. J Biol Chem. 1972 Nov 25;247(22):7407–7419. [PubMed] [Google Scholar]
  11. Oaks A., Jones K., Misra S. A comparison of glutamate synthase obtained from maize endosperms and roots. Plant Physiol. 1979 May;63(5):793–795. doi: 10.1104/pp.63.5.793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rathnam C. K., Edwards G. E. Distribution of Nitrate-assimilating Enzymes between Mesophyll Protoplasts and Bundle Sheath Cells in Leaves of Three Groups of C(4) Plants. Plant Physiol. 1976 Jun;57(6):881–885. doi: 10.1104/pp.57.6.881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Robertson J. G., Warburton M. P., Farnden K. J. Induction of glutamate synthase during nodule development in lupin. FEBS Lett. 1975 Jul 15;55(1):33–37. doi: 10.1016/0014-5793(75)80950-1. [DOI] [PubMed] [Google Scholar]
  14. Scopes R. K. Measurement of protein by spectrophotometry at 205 nm. Anal Biochem. 1974 May;59(1):277–282. doi: 10.1016/0003-2697(74)90034-7. [DOI] [PubMed] [Google Scholar]
  15. Sodek L., da Silva W. J. Glutamate synthase: a possible role in nitrogen metabolism of the developing maize endosperm. Plant Physiol. 1977 Oct;60(4):602–605. doi: 10.1104/pp.60.4.602. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Suzuki A., Gadal P. Glutamate synthase from rice leaves. Plant Physiol. 1982 Apr;69(4):848–852. doi: 10.1104/pp.69.4.848. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tempest D. W., Meers J. L., Brown C. M. Synthesis of glutamate in Aerobacter aerogenes by a hitherto unknown route. Biochem J. 1970 Apr;117(2):405–407. doi: 10.1042/bj1170405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES