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. 1983 Feb;71(2):420–424. doi: 10.1104/pp.71.2.420

Cytochemical and Cytofluorometric Evidence for Guard Cell Photosystems 1

Kevin C Vaughn 1,2, William H Outlaw Jr 1,2
PMCID: PMC1066047  PMID: 16662840

Abstract

Evidence for photosynthetic linear electron transport in guard cells was obtained with two sensitive methods of high spacial resolution. Light-dependent diaminobenzidine oxidation (an indicator of PSI) and DCMU-sensitive, light-dependent thiocarbamyl nitroblue tetrazolium reduction (an indicator of PSII) were observed in guard cell plastids of Hordeum vulgare L. cv Himalaya using electron microscopic cytochemical procedures. DCMU-sensitive Chl a fluorescence induction (an indicator of PSII) was detected in individual guard cell pairs of Vicia faba L. cv Longpod using an ultramicrofluorometer. At least for these species, we conclude these results are proof for the presence of PSII in guard cell chloroplasts, which until now has been somewhat controversial.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chua N. H. Photooxidation of 3,3'-diaminobenzidine by blue-green algae and Chlamydomonas reinhardii. Biochim Biophys Acta. 1972 Apr 20;267(1):179–189. doi: 10.1016/0005-2728(72)90149-1. [DOI] [PubMed] [Google Scholar]
  2. FREELAND R. O. The green pigment and physiology of guard cells. Science. 1951 Jul 27;114(2952):94–95. doi: 10.1126/science.114.2952.94. [DOI] [PubMed] [Google Scholar]
  3. Kalina M., Plapinger R. E., Hoshino Y., Seligman A. M. Nonosmiophilic tetrazolium salts that yield osmiophilic, lipophobic formazans for ultrastructural localization of dehydrogenase activity. J Histochem Cytochem. 1972 Sep;20(9):685–695. doi: 10.1177/20.9.685. [DOI] [PubMed] [Google Scholar]
  4. Marty D. Localisation ultra-structurale des sites d'activité des photosystèmes I et II dans les chloroplastes in situ. C R Acad Sci Hebd Seances Acad Sci D. 1977 Jul 4;285(1):27–30. [PubMed] [Google Scholar]
  5. Melis A., Zeiger E. Chlorophyll a Fluorescence Transients in Mesophyll and Guard Cells : MODULATION OF GUARD CELL PHOTOPHOSPHORYLATION BY CO(2). Plant Physiol. 1982 Mar;69(3):642–647. doi: 10.1104/pp.69.3.642. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Nir I., Pease D. C. Chloroplast organization and the ultrastructural localization of photosystems I and II. J Ultrastruct Res. 1973 Mar;42(5):534–550. doi: 10.1016/s0022-5320(73)80025-5. [DOI] [PubMed] [Google Scholar]
  7. Ogawa T., Grantz D., Boyer J., Govindjee Effects of Cations and Abscisic Acid on Chlorophyll a Fluorescence in Guard Cells of Vicia faba. Plant Physiol. 1982 May;69(5):1140–1144. doi: 10.1104/pp.69.5.1140. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Outlaw W. H., Manchester J., Dicamelli C. A., Randall D. D., Rapp B., Veith G. M. Photosynthetic carbon reduction pathway is absent in chloroplasts of Vicia faba guard cells. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6371–6375. doi: 10.1073/pnas.76.12.6371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Outlaw W. H., Mayne B. C., Zenger V. E., Manchester J. Presence of Both Photosystems in Guard Cells of Vicia faba L: IMPLICATIONS FOR ENVIRONMENTAL SIGNAL PROCESSING. Plant Physiol. 1981 Jan;67(1):12–16. doi: 10.1104/pp.67.1.12. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Outlaw W. H., Tarczynski M. C., Anderson L. C. Taxonomic survey for the presence of ribulose-1,5-bisphosphate carboxylase activity in guard cells. Plant Physiol. 1982 Oct;70(4):1218–1220. doi: 10.1104/pp.70.4.1218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pallas J. E. Photophosphorylation Can Provide Sufficient Adenosine 5'-Triphosphate to Drive K Movements during Stomatal Opening. Plant Physiol. 1972 Apr;49(4):649–650. doi: 10.1104/pp.49.4.649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sharkey T. D., Raschke K. Effect of Light Quality on Stomatal Opening in Leaves of Xanthium strumarium L. Plant Physiol. 1981 Nov;68(5):1170–1174. doi: 10.1104/pp.68.5.1170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Willmer C. M., Pallas J. E., Black C. C. Carbon dioxide metabolism in leaf epidermal tissue. Plant Physiol. 1973 Nov;52(5):448–452. doi: 10.1104/pp.52.5.448. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Zeiger E., Armond P., Melis A. Fluorescence Properties of Guard Cell Chloroplasts: EVIDENCE FOR LINEAR ELECTRON TRANSPORT AND LIGHT-HARVESTING PIGMENTS OF PHOTOSYSTEMS I AND II. Plant Physiol. 1981 Jan;67(1):17–20. doi: 10.1104/pp.67.1.17. [DOI] [PMC free article] [PubMed] [Google Scholar]

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