Abstract
Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCase) was isolated from isogenic diploid-tetraploid and tetraploid-octoploid sets of alfalfa (Medicago sativa L.) leaves. Molecular weights of RuBPCase subunits were similar across ploidy levels of both isogenic sets with subunits of 52,000 and 14,000. Apparent Km(CO2) values and substrate specificity factors (VcKo/VoKc) of RuBPCase were similar across ploidy levels of both isogenic sets. These results indicate that ploidy had no effect on the kinetic properties of RuBPCase in alfalfa.
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- Chua N. H., Schmidt G. W. Post-translational transport into intact chloroplasts of a precursor to the small subunit of ribulose-1,5-bisphosphate carboxylase. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6110–6114. doi: 10.1073/pnas.75.12.6110. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gottlieb L. D. Conservation and duplication of isozymes in plants. Science. 1982 Apr 23;216(4544):373–380. doi: 10.1126/science.216.4544.373. [DOI] [PubMed] [Google Scholar]
- Jordan D. B., Ogren W. L. A Sensitive Assay Procedure for Simultaneous Determination of Ribulose-1,5-bisphosphate Carboxylase and Oxygenase Activities. Plant Physiol. 1981 Feb;67(2):237–245. doi: 10.1104/pp.67.2.237. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Joseph M. C., Randall D. D. Photosynthesis in Polyploid Tall Fescue : II. PHOTOSYNTHESIS AND RIBULOSE-1, 5-BISPHOSPHATE CARBOXYLASE OF POLYPLOID TALL FESCUE. Plant Physiol. 1981 Oct;68(4):894–898. doi: 10.1104/pp.68.4.894. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kung S. D., Sakano K., Wildman S. G. Multiple peptide composition of the large and small subunits of Nicotiana tabacum fraction I protein ascertained by fingerprinting and electrofocusing. Biochim Biophys Acta. 1974 Sep 13;365(1):138–147. doi: 10.1016/0005-2795(74)90258-x. [DOI] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- McNeil P. H., Foyer C. H., Walker D. A. Similarity of Ribulose-1,5-bisphosphate Carboxylases of Isogenic Diploid and Tetraploid Ryegrass (Lolium perenne L.) Cultivars. Plant Physiol. 1981 Mar;67(3):530–534. doi: 10.1104/pp.67.3.530. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Merril C. R., Goldman D., Sedman S. A., Ebert M. H. Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science. 1981 Mar 27;211(4489):1437–1438. doi: 10.1126/science.6162199. [DOI] [PubMed] [Google Scholar]
- Meyers S. P., Nichols S. L., Baer G. R., Molin W. T., Schrader L. E. Ploidy effects in isogenic populations of alfalfa : I. Ribulose-1,5-bisphosphate carboxylase, soluble protein, chlorophyll, and DNA in leaves. Plant Physiol. 1982 Dec;70(6):1704–1709. doi: 10.1104/pp.70.6.1704. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Molin W. T., Meyers S. P., Baer G. R., Schrader L. E. Ploidy Effects in Isogenic Populations of Alfalfa : II. Photosynthesis, Chloroplast Number, Ribulose-1,5-Bisphosphate Carboxylase, Chlorophyll, and DNA in Protoplasts. Plant Physiol. 1982 Dec;70(6):1710–1714. doi: 10.1104/pp.70.6.1710. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rathnam C. K., Chollet R. Photosynthetic and Photorespiratory Carbon Metabolism in Mesophyll Protoplasts and Chloroplasts Isolated from Isogenic Diploid and Tetraploid Cultivars of Ryegrass (Lolium perenne L.). Plant Physiol. 1980 Mar;65(3):489–494. doi: 10.1104/pp.65.3.489. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rejda J. M., Johal S., Chollet R. Enzymic and physicochemical characterization of ribulose 1,5-bisphosphate carboxylase/oxygenase from diploid and tetraploid cultivars of perennial ryegrass. Arch Biochem Biophys. 1981 Sep;210(2):617–624. doi: 10.1016/0003-9861(81)90228-9. [DOI] [PubMed] [Google Scholar]
- Timko M. P., Vasconcelos A. C., Fairbrothers D. E. Euploidy in Ricinus. I. Euploidy and gene dosage effects on cellular proteins. Biochem Genet. 1980 Feb;18(1-2):171–183. doi: 10.1007/BF00504367. [DOI] [PubMed] [Google Scholar]
- Tomimatsu Y. Macromolecular properties and subunit interactions of ribulose-1,5-bisphosphate carboxylase from alfalfa. Biochim Biophys Acta. 1980 Mar 26;622(1):85–93. doi: 10.1016/0005-2795(80)90160-9. [DOI] [PubMed] [Google Scholar]