Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1983 May;72(1):245–250. doi: 10.1104/pp.72.1.245

Gas Exchange, Stomatal Behavior, and δ13C Values of the flacca Tomato Mutant in Relation to Abscisic Acid 1

Kent J Bradford 1,2, Thomas D Sharkey 1,3, Graham D Farquhar 1
PMCID: PMC1066203  PMID: 16662969

Abstract

The relationship between stomatal conductance and capacity for assimilation was investigated in flacca, a mutant of tomato (Lycopersicon esculentum Mill.) that has abnormal stomatal behavior and low abscisic acid (ABA) content. The assimilation capacity, determined by measuring assimilation rate as a function of intercellular CO2 pressure, did not differ in leaves of flacca and its parent variety, Rheinlands Ruhm (RR). On the other hand, stomatal conductance of flacca leaves was greater than that of RR, and could be phenotypically reverted by spraying with 30 micromolar ABA. Stomatal conductance of flacca leaves was also reduced by increasing CO2 pressure, increasing leaf to air vapor pressure difference, and decreasing quantum flux, irrespective of ABA treatment.

The high conductance of flacca leaves resulted in a high intercellular CO2 pressure. This allowed greater discrimination against 13CO2, as evidenced by more negative δ 13C values for flacca as compared to RR. The δ 13C values of both flacca and RR plants as influenced by ABA treatment were consistent with predictions based on gas exchange measurements, using a recent model of discrimination.

Full text

PDF
247

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradford K. J. Water Relations and Growth of the flacca Tomato Mutant in Relation to Abscisic Acid. Plant Physiol. 1983 May;72(1):251–255. doi: 10.1104/pp.72.1.251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dubbe D. R., Farquhar G. D., Raschke K. Effect of abscisic Acid on the gain of the feedback loop involving carbon dioxide and stomata. Plant Physiol. 1978 Sep;62(3):413–417. doi: 10.1104/pp.62.3.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Imber D., Tal M. Phenotypic reversion of flacca, a wilty mutant of tomato, by abscisic Acid. Science. 1970 Aug 7;169(3945):592–593. doi: 10.1126/science.169.3945.592. [DOI] [PubMed] [Google Scholar]
  4. Mawson B. T., Colman B., Cummins W. R. Abscisic Acid and photosynthesis in isolated leaf mesophyll cell. Plant Physiol. 1981 Feb;67(2):233–236. doi: 10.1104/pp.67.2.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Pallas J. E., Jr Transpiration and Stomatal Opening with Changes in Carbon Dioxide Content of the Air. Science. 1965 Jan 8;147(3654):171–173. doi: 10.1126/science.147.3654.171. [DOI] [PubMed] [Google Scholar]
  6. Sharkey T. D., Raschke K. Effects of phaseic Acid and dihydrophaseic Acid on stomata and the photosynthetic apparatus. Plant Physiol. 1980 Feb;65(2):291–297. doi: 10.1104/pp.65.2.291. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Sharkey T. D., Raschke K. Separation and measurement of direct and indirect effects of light on stomata. Plant Physiol. 1981 Jul;68(1):33–40. doi: 10.1104/pp.68.1.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Tal M. Abnormal Stomatal Behavior and Hormonal Imbalance in flacca, a Wilty Mutant of Tomato: V. Effect of Abscisic Acid on Indoleacetic Acid Metabolism and Ethylene Evolution. Plant Physiol. 1979 Jun;63(6):1044–1048. doi: 10.1104/pp.63.6.1044. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Tal M. Abnormal stomatal behavior in wilty mutants of tomato. Plant Physiol. 1966 Oct;41(8):1387–1391. doi: 10.1104/pp.41.8.1387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Tal M., Imber D., Itai C. Abnormal Stomatal Behavior and Hormonal Imbalance in flacca, a Wilty Mutant of Tomato: I. Root Effect and Kinetin-like Activity. Plant Physiol. 1970 Sep;46(3):367–372. doi: 10.1104/pp.46.3.367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Tal M., Nevo Y. Abnormal stomatal behavior and root resistance, and hormonal imbalance in three wilty mutants of tomato. Biochem Genet. 1973 Mar;8(3):291–300. doi: 10.1007/BF00486182. [DOI] [PubMed] [Google Scholar]
  12. Wong S. C., Cowan I. R., Farquhar G. D. Leaf Conductance in Relation to Assimilation in Eucalyptus pauciflora Sieb. ex Spreng: Influence of Irradiance and Partial Pressure of Carbon Dioxide. Plant Physiol. 1978 Oct;62(4):670–674. doi: 10.1104/pp.62.4.670. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES