Skip to main content
Plant Physiology logoLink to Plant Physiology
. 1983 Aug;72(4):1127–1129. doi: 10.1104/pp.72.4.1127

Subcellular Localization of Asparaginase and Asparagine Aminotransferase in Pisum sativum Leaves 1

Robert J Ireland 1, Kenneth W Joy 1
PMCID: PMC1066386  PMID: 16663132

Abstract

Protoplasts isolated from young and mature pea leaves (Pisum sativum L.) were broken and their contents fractionated by differential centrifugation or on sucrose-density gradients. Asparaginase was found only in the cytosol of young leaves. Asparagine aminotransferase was found in both young and mature leaves and was localized exclusively in the peroxisome. This corroborates the observation that asparagine transamination is catalyzed by the serine:glyoxylate aminotransferase.

Full text

PDF
1129

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cooper A. J. Asparagine transaminase from rat liver. J Biol Chem. 1977 Mar 25;252(6):2032–2038. [PubMed] [Google Scholar]
  2. Ireland R. J., Joy K. W. Purification and properties of an asparagine aminotransferase from Pisum sativum leaves. Arch Biochem Biophys. 1983 May;223(1):291–296. doi: 10.1016/0003-9861(83)90594-5. [DOI] [PubMed] [Google Scholar]
  3. KRETOVICH W. L. The biosynthesis of dicarboxylic amino acids and enzymic transformations of amides in plants. Adv Enzymol Relat Subj Biochem. 1958;20:319–340. doi: 10.1002/9780470122655.ch10. [DOI] [PubMed] [Google Scholar]
  4. Lloyd N. D., Joy K. W. 2-Hydroxysuccinamic acid: a product of asparagine metabolis in plants. Biochem Biophys Res Commun. 1978 Mar 15;81(1):186–192. doi: 10.1016/0006-291x(78)91647-9. [DOI] [PubMed] [Google Scholar]
  5. MEISTER A., SOBER H. A., TICE S. V., FRASER P. E. Transamination and associated deamidation of asparagine and glutamine. J Biol Chem. 1952 May;197(1):319–330. [PubMed] [Google Scholar]
  6. RACKER E. Spectrophotometric measurements of the enzymatic formation of fumaric and cis-aconitic acids. Biochim Biophys Acta. 1950 Jan;4(1-3):211–214. doi: 10.1016/0006-3002(50)90026-6. [DOI] [PubMed] [Google Scholar]
  7. Rehfeld D. W., Tolbert N. E. Aminotransferases in peroxisomes from spinach leaves. J Biol Chem. 1972 Aug 10;247(15):4803–4811. [PubMed] [Google Scholar]
  8. Sodek L. Distribution and Properties of a Potassium-dependent Asparaginase Isolated from Developing Seeds of Pisum sativum and Other Plants. Plant Physiol. 1980 Jan;65(1):22–26. doi: 10.1104/pp.65.1.22. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Streeter J. G. Asparaginase and asparagine transaminase in soybean leaves and root nodules. Plant Physiol. 1977 Aug;60(2):235–239. doi: 10.1104/pp.60.2.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Wallsgrove R. M., Lea P. J., Miflin B. J. Distribution of the Enzymes of Nitrogen Assimilation within the Pea Leaf Cell. Plant Physiol. 1979 Feb;63(2):232–236. doi: 10.1104/pp.63.2.232. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Wintermans J. F., de Mots A. Spectrophotometric characteristics of chlorophylls a and b and their pheophytins in ethanol. Biochim Biophys Acta. 1965 Nov 29;109(2):448–453. doi: 10.1016/0926-6585(65)90170-6. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES