Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1983 Sep;73(1):100–104. doi: 10.1104/pp.73.1.100

Short Term Studies of Nitrate Uptake into Barley Plants Using Ion-Specific Electrodes and 36ClO3−1

I. Control of Net Uptake by NO3− Efflux

Celia E Deane-Drummond 1,2, Anthony D M Glass 1
PMCID: PMC1066415  PMID: 16663154

Abstract

A computer-controlled multichannel data acquisition system was employed to obtain continuous measurements of net nitrate or chlorate uptake by roots of intact barley plants (Hordeum vulgare cv Betzes) using nitrate-specific electrodes. Plants, previously grown in solutions maintained at 10 or 200 micromolar NO3− (low N or high N conditions, respectively), were provided with 200 micromolar NO3− or ClO3− during the uptake period. Initial rates of NO3− uptake were several times higher in low N plants than in high N plants. Within 10 min, uptake in the former plants declined to a new steady rate which was sustained for the remainder of the experiment. No such time-dependent changes were evident in the high N plants. Rates and patterns of net chlorate uptake exhibited almost identical dependence upon previous nitrate provision. NO3− (36ClO3−) influx, by contrast, appeared to be independent of NO3− pretreatment prior to influx determination. Nitrate efflux, estimated by several different methods, was strongly correlated with internal nitrate concentration of the roots.

Full text

PDF
103

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Deane-Drummond C. E., Glass A. D. Nitrate Uptake into Barley (Hordeum vulgare) Plants : A New Approach Using ClO(3) as an Analog for NO(3). Plant Physiol. 1982 Jul;70(1):50–54. doi: 10.1104/pp.70.1.50. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Glass A. The regulation of potassium absorption in barley roots. Plant Physiol. 1975 Sep;56(3):377–380. doi: 10.1104/pp.56.3.377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. JACKSON P. C., ADAMS H. R. Cation-anion balance during potassium and sodium absorption by barley roots. J Gen Physiol. 1963 Jan;46:369–386. doi: 10.1085/jgp.46.3.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. John P. Aerobic and anaerobic bacterial respiration monitored by electrodes. J Gen Microbiol. 1977 Jan;98(1):231–238. doi: 10.1099/00221287-98-1-231. [DOI] [PubMed] [Google Scholar]
  5. Mackown C. T., Volk R. J., Jackson W. A. Nitrate Accumulation, Assimilation, and Transport by Decapitated Corn Roots : EFFECTS OF PRIOR NITRATE NUTRITION. Plant Physiol. 1981 Jul;68(1):133–138. doi: 10.1104/pp.68.1.133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Morgan M. A., Volk R. J., Jackson W. A. Simultaneous Influx and Efflux of Nitrate during Uptake by Perennial Ryegrass. Plant Physiol. 1973 Feb;51(2):267–272. doi: 10.1104/pp.51.2.267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Reed A. J., Hageman R. H. Relationship between Nitrate Uptake, Flux, and Reduction and the Accumulation of Reduced Nitrogen in Maize (Zea mays L.): I. GENOTYPIC VARIATION. Plant Physiol. 1980 Dec;66(6):1179–1183. doi: 10.1104/pp.66.6.1179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Rufty T. W., Jackson W. A., Raper C. D. Nitrate Reduction in Roots as Affected by the Presence of Potassium and by Flux of Nitrate through the Roots. Plant Physiol. 1981 Sep;68(3):605–609. doi: 10.1104/pp.68.3.605. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES