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. 1984 Feb;74(2):239–246. doi: 10.1104/pp.74.2.239

Phytohormones, Rhizobium Mutants, and Nodulation in Legumes 1

V. Cytokinin Metabolism in Effective and Ineffective Pea Root Nodules

Jane Badenoch-Jones 1,2, Barry G Rolfe 1, David S Letham 1
PMCID: PMC1066662  PMID: 16663404

Abstract

[3H]Zeatin riboside was supplied to intact pea (Pisum sativum) plants either onto the leaves or onto the root nodules. When applied directly to nodules, approximately 70% of recovered radioactivity remained in the nodules, approximately 15% was detected in the root system, and 15% was in the shoot. However, when supplied to the leaves, little 3H was transported, with approximately 0.05% of recovered radioactivity being found in the root system and nodules. On a fresh weight basis, nodules accumulated more 3H than the parent root. In both types of studies, metabolites with an intact zeatin moiety were detected in root nodules.

In all experiments, two-dimensional thin layer chromatography revealed that little 3H remained as zeatin riboside in root or nodule tissue at the end of the labeling period. Nodules metabolized [3H]zeatin riboside to the following cytokinins/cytokinin metabolites: zeatin, adenosine, adenine, the O-glucosides of zeatin and zeatin riboside, lupinic acid, nucleotides of adenine and zeatin, and the dihydro derivatives of many of these compounds.

Although a few small differences were observed, there were no major differences between root and nodule tissue in their metabolism of [3H] zeatin riboside. Furthermore, any differences between effective and ineffective nodules were generally minor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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