Skip to main content
Plant Physiology logoLink to Plant Physiology
. 1984 May;75(1):203–206. doi: 10.1104/pp.75.1.203

Changes in Levels of α-Amylase Components in Barley Tissues during Germination and Early Seedling Growth 1

Alexander W MacGregor 1,2, F Helen MacDougall 1,2, Christiane Mayer 1,2, Jean Daussant 1,2
PMCID: PMC1066862  PMID: 16663571

Abstract

Kernels of Klages barley (Hordeum vulgare L.) were germinated for 1 to 4 days on moist sand at 18°C. Representative kernels from each time period were dissected to give the following fractions: scutellum, subscutellar endosperm, aleurone-scutellum interface, remaining aleurone, subaleurone endosperm, and core endosperm. These tissues were analyzed for α-amylase components by isoelectric focusing and rocket-line immunoelectrophoresis. Although aleurone and scutellar tissues appeared to synthesize the same α-amylase components, enzyme was detected first in the scutellum. A larger proportion of scutellar α-amylase was excreted into the endosperm compared to aleurone synthesized α-amylase. Aleurone cells appeared to synthesize appreciably more α-amylase than did scutellar tissue.

Full text

PDF
205

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bog-Hansen T. C., Daussant J. Immunochemical quantitation of isoenzymes. Alpha-amylase isoenzymes in barley malt. Anal Biochem. 1974 Oct;61(2):522–527. doi: 10.1016/0003-2697(74)90420-5. [DOI] [PubMed] [Google Scholar]
  2. Callis J., Ho T. H. Multiple molecular forms of the gibberellin-induced alpha-amylase from the aleurone layers of barley seeds. Arch Biochem Biophys. 1983 Jul 1;224(1):224–234. doi: 10.1016/0003-9861(83)90206-0. [DOI] [PubMed] [Google Scholar]
  3. Chrispeels M. J., Varner J. E. Gibberellic Acid-enhanced synthesis and release of alpha-amylase and ribonuclease by isolated barley and aleurone layers. Plant Physiol. 1967 Mar;42(3):398–406. doi: 10.1104/pp.42.3.398. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Coombe B. G., Cohen D., Paleg L. G. Barley endosperm bioassay for gibberellins. I. Parameters of the response system. Plant Physiol. 1967 Jan;42(1):105–112. doi: 10.1104/pp.42.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kroll J. Rocket-line immunoelectrophoresis (76). Scand J Immunol Suppl. 1973;1:83–87. [PubMed] [Google Scholar]
  6. Weselake R. J., Macgregor A. W., Hill R. D. An endogenous alpha-amylase inhibitor in barley kernels. Plant Physiol. 1983 Jul;72(3):809–812. doi: 10.1104/pp.72.3.809. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES