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. 1982 Aug;70(2):560–566. doi: 10.1104/pp.70.2.560

A Comparison of Soybean Agglutinin in Cultivars Resistant and Susceptible to Phytophthora megasperma var. sojae (Race 1) 1

Donna M Gibson 1,2, Sharon Stack 1,3, Kathryne Krell 1,3, Jan House 1,3
PMCID: PMC1067188  PMID: 16662534

Abstract

The amount of soybean agglutinin (SBA) detectable by radioimmunoassay in seeds of resistant cultivars to Phytophthora megasperma var. sojae was approximately twice that of susceptible cultivars. SBA was preferentially released at earlier times (6-9 hours) and in higher amounts in the imbibate from resistant cultivars as compared to susceptible cultivars. The lectin in the imbibate was immunologically identical to the seed lectin, indicating little or no proteolysis had occurred, and was active in hemagglutination. Binding of fluorescein isothiocyanate-labeled SBA to mycelial cell walls could be abolished by adding N-acetyl galactosamine or galactose. Purified SBA at concentrations of 150 to 300 micrograms inhibited mycelial growth by 50%, and the imbibate from Govan (resistant) cultivar was more inhibitory than the imbibate from Shore (susceptible) cultivar. Removal of SBA from the imbibate by affinity chromatography abolished the inhibition of mycelial growth, but the inhibition could be recovered from the eluant containing lectin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bohlool B. B., Schmidt E. L. Lectins: a possible basis for specificity in the Rhizobium--legume root nodule symbiosis. Science. 1974 Jul 19;185(4147):269–271. doi: 10.1126/science.185.4147.269. [DOI] [PubMed] [Google Scholar]
  2. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  3. Fountain D. W., Foard D. E., Replogle W. D., Yang W. K. Lectin release by soybean seeds. Science. 1977 Sep 16;197(4309):1185–1187. doi: 10.1126/science.197.4309.1185. [DOI] [PubMed] [Google Scholar]
  4. Gade W., Jack M. A., Dahl J. B., Schmidt E. L., Wold F. The isolation and characterization of a root lectin from soybean (Glycine max (L), cultivar Chippewa). J Biol Chem. 1981 Dec 25;256(24):12905–12910. [PubMed] [Google Scholar]
  5. Goldstein I. J., Hayes C. E. The lectins: carbohydrate-binding proteins of plants and animals. Adv Carbohydr Chem Biochem. 1978;35:127–340. doi: 10.1016/s0065-2318(08)60220-6. [DOI] [PubMed] [Google Scholar]
  6. Hammarström S., Murphy L. A., Goldstein I. J., Etzler M. E. Carbohydrate binding specificity of four N-acetyl-D-galactosamine- "specific" lectins: Helix pomatia A hemagglutinin, soy bean agglutinin, lima bean lectin, and Dolichos biflorus lectin. Biochemistry. 1977 Jun 14;16(12):2750–2755. doi: 10.1021/bi00631a025. [DOI] [PubMed] [Google Scholar]
  7. Keen N. T. Specific elicitors of plant phytoalexin production: detenninants of race specificity in pathogens? Science. 1975 Jan 10;187(4171):74–75. doi: 10.1126/science.187.4171.74. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Lis H., Sharon N., Katchalski E. Soybean hemagglutinin, a plant glycoprotein. I. Isolation of a glycopeptide. J Biol Chem. 1966 Feb 10;241(3):684–689. [PubMed] [Google Scholar]
  10. Mirelman D., Galun E., Sharon N., Lotan R. Inhibition of fungal growth by wheat germ agglutinin. Nature. 1975 Jul 31;256(5516):414–416. doi: 10.1038/256414a0. [DOI] [PubMed] [Google Scholar]
  11. Mishkind M., Keegstra K., Palevitz B. A. Distribution of wheat germ agglutinin in young wheat plants. Plant Physiol. 1980 Nov;66(5):950–955. doi: 10.1104/pp.66.5.950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pull S. P., Pueppke S. G., Hymowitz T., Orf J. H. Soybean lines lacking the 120,000-dalton seed lectin. Science. 1978 Jun 16;200(4347):1277–1279. doi: 10.1126/science.200.4347.1277. [DOI] [PubMed] [Google Scholar]
  13. RINDERKNECHT H. Ultra-rapid fluorescent labelling of proteins. Nature. 1962 Jan 13;193:167–168. doi: 10.1038/193167b0. [DOI] [PubMed] [Google Scholar]
  14. Rice R. H., Means G. E. Radioactive labeling of proteins in vitro. J Biol Chem. 1971 Feb 10;246(3):831–832. [PubMed] [Google Scholar]
  15. Uy R., Wold F. 1,4-Butanediol diglycidyl ether coupling of carbohydrates to Sepharose: affinity adsorbents for lectins and glycosidases. Anal Biochem. 1977 Jul;81(1):98–107. doi: 10.1016/0003-2697(77)90602-9. [DOI] [PubMed] [Google Scholar]

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