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. 1982 Aug;70(2):585–589. doi: 10.1104/pp.70.2.585

Inhibition by Adenine Derivatives of the Cyanide-Insensitive Electron Transport Pathway of Plant Mitochondria

Pierre Dizengremel 1, Michèle Chauveau 1, Jean Roussaux 1
PMCID: PMC1067192  PMID: 16662538

Abstract

The effect of benzylaminopurine was studied on cyanide-resistant mitochondria isolated from aged slices of potato tuber (Solanum tuberosum L. var. Bintje). Benzylaminopurine specifically acted on the cyanide-resistant alternative pathway. In the case of succinate oxidation, it mimicked the action of salicylhydroxamic acid and restored a good oxidative phosphorylation. Kinetic analyses showed that inhibitions by benzylaminopurine, salicylhydroxamic acid, and disulfiram occurred at mutually exclusive sites on the alternative pathway. Cyanide-resistant malate oxidation was only partially inhibited by benzylaminopurine and this inhibition occurred for low concentrations of this compound. On the other hand, the oxidation of exogenous NADH remained unaffected.

The effects of several adenine derivatives with or without cytokinin activity and that of a purine analog with anticytokinin activity were also studied. The variation in effectiveness to inhibit cyanide-resistant electron transport was: benzylaminopurine and 7-pentylamino-3-methylpyrazolo (4,3 d) pyrimidine (anticytokinin) > α-α′ dimethyl-allyl-adenine > 6-benzoylamino-9-benzylpurine > kinetin > adenine. No correlation was observed between the ability to inhibit the alternative pathway and the biological activity of these compounds. Liposolubility appeared as a major factor for potential inhibitory effect on the alternative pathway.

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Selected References

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  1. CANNON C., COWAN F. F., KOPPANYI T., MAENGWYN-DAVIES G. D. Explanation of cocaine desensitization of blood pressure responses to ephedrine. Science. 1961 Oct 13;134(3485):1075–1077. doi: 10.1126/science.134.3485.1075-a. [DOI] [PubMed] [Google Scholar]
  2. DIXON M. The determination of enzyme inhibitor constants. Biochem J. 1953 Aug;55(1):170–171. doi: 10.1042/bj0550170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dizengremel P., Lance C. Control of Changes in Mitochondrial Activities during Aging of Potato Slices. Plant Physiol. 1976 Aug;58(2):147–151. doi: 10.1104/pp.58.2.147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gregorini G., Laloue M. Biological Effects of Cytokinin Antagonists 7-(Pentylamino) and 7-(Benzylamino)-3-Methylpyrazolo(4,3-d)Pyrimidines on Suspension-cultured Tobacco Cells. Plant Physiol. 1980 Feb;65(2):363–367. doi: 10.1104/pp.65.2.363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Grover S. D., Laties G. G. Disulfiram inhibition of the alternative respiratory pathway in plant mitochondria. Plant Physiol. 1981 Aug;68(2):393–400. doi: 10.1104/pp.68.2.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hackett D. P., Haas D. W., Griffiths S. K., Niederpruem D. J. Studies on Development of Cyanide-resistant Respiration in Potato Tuber Slices. Plant Physiol. 1960 Jan;35(1):8–19. doi: 10.1104/pp.35.1.8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Miller C. O. Cytokinin inhibition of respiration in mitochondria from six plant species. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4731–4735. doi: 10.1073/pnas.77.8.4731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Moore T. S., Miller C. O. Effects of cytokinins on the respiration of soybean callus tissue. Plant Physiol. 1972 Nov;50(5):594–598. doi: 10.1104/pp.50.5.594. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Palmer J. M. The influence of growth regulating substances on the development of enhanced metabolic rates in thin slices of beetroot storage tissue. Plant Physiol. 1966 Sep;41(7):1173–1178. doi: 10.1104/pp.41.7.1173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rustin P., Moreau F., Lance C. Malate Oxidation in Plant Mitochondria via Malic Enzyme and the Cyanide-insensitive Electron Transport Pathway. Plant Physiol. 1980 Sep;66(3):457–462. doi: 10.1104/pp.66.3.457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schonbaum G. R., Bonner W. D., Jr, Storey B. T., Bahr J. T. Specific inhibition of the cyanide-insensitive respiratory pathway in plant mitochondria by hydroxamic acids. Plant Physiol. 1971 Jan;47(1):124–128. doi: 10.1104/pp.47.1.124. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Siedow J. N., Girvin M. E. Alternative Respiratory Pathway: ITS ROLE IN SEED RESPIRATION AND ITS INHIBITION BY PROPYL GALLATE. Plant Physiol. 1980 Apr;65(4):669–674. doi: 10.1104/pp.65.4.669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. THIMANN K. V., YOCUM C. S., HACKETT D. P. Terminal oxidases and growth in plant tissues. III. Terminal oxidation in potato tuber tissue. Arch Biochem Biophys. 1954 Nov;53(1):239–257. doi: 10.1016/0003-9861(54)90249-0. [DOI] [PubMed] [Google Scholar]

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