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. 1982 Aug;70(2):616–625. doi: 10.1104/pp.70.2.616

Influence of Nitrate and Ammonia on Photosynthetic Characteristics and Leaf Anatomy of Moricandia arvensis1

Klaus Winter 1,2,3,2, Hideaki Usuda 1,2,3,3, Mikio Tsuzuki 1,2,3,4, Mark Schmitt 1,2,3,5, Gerald E Edwards 1,2,3,5, Richard J Thomas 1,2,3, Ray F Evert 1,2,3
PMCID: PMC1067198  PMID: 16662544

Abstract

The leaf anatomy and certain photosynthetic properties of nitrate- and ammonia-grown plants of Moricandia arvensis (L.) DC., a species previously reported to be a C3-C4 intermediate, were investigated. Nitrate-grown plants had a high level of malate in the leaves while ammonia-grown plants had low levels of malate. In young leaves of nitrate-grown plants, there was a diurnal fluctuation of malate content, increasing during the day and decreasing during the night. Titratable acidity remained low in leaves of both nitrate- and ammonia-grown plants.

In nitrate-grown plants, the activity of phosphoenolpyruvate (PEP) carboxylase was about 2-fold higher than in ammonia-grown plants, the latter having activity typical of C3 species. Also, in nitrate-grown plants, the ratio of activities of ribulose 1,5-bisphosphate (RuBP) carboxylase/PEP carboxylase was lower than in ammonia-grown plants. Nitrate reductase activities were higher in nitrate- than in ammonia-grown plants and the greatest activity was found in younger leaves.

With nitrate-grown plants, during a pulse-chase experiment the label in malate, as a percentage of the total labeled products, increased from about 7% after a 10-second pulse with 14CO2 up to 17% during a 5-minute chase with 12CO2. The pattern of 14C labeling in various metabolites suggests the primary carboxylation is through RuBP carboxylase with a secondary carboxylation through PEP carboxylase. In similar experiments, with ammonia-grown plants, the percentage label in malate was only 0% to 4% with no increase in malate labeling during the chase period. The CO2 compensation point was lower in nitrate-grown than ammonia-grown plants.

There was no evidence of Kranz-like anatomy in either the nitrate or ammonia-grown plants. Mitochondria of bundle-sheath cells were strikingly positioned along the inner tangential wall. This might allow the chloroplasts of these cells to fix the mitochondrial photorespired CO2 more effectively and contribute to the low CO2 compensation point in the species. Chloroplasts of bundle-sheath cells and contiguous mesophyll cells were similar in size and structure in plants grown on different media, although chloroplast thylakoids and stromata of the ammonia-grown plants stained more intensely than those of nitrate-grown plants. In addition, irregular clusters of phytoferritin particles occurred in the chloroplasts of the ammonia-grown plants.

The results indicate that the substantial activity of PEP carboxylase, incorporation of CO2 into malate, the high malate content, and in part the relatively low CO2 compensation point in Moricandia arvensis may be accounted for by metabolism of nitrate rather than by a state of C3-C4 intermediacy.

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