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. 1985 Oct;79(2):568–570. doi: 10.1104/pp.79.2.568

High Efficiency Transformation of Cultured Tobacco Cells 1

Gynheung An 1
PMCID: PMC1074928  PMID: 16664453

Abstract

Tobacco calli were transformed at levels up to 50% by cocultivation of tobacco cultured cells with Agrobacterium tumefaciens harboring the binary transfer-DNA vector, pGA472, containing a kanamycin resistance marker. Transformation frequency was dependent on the physiological state of the tobacco cells, the nature of Agrobacterium strain and, less so, on the expression of the vir genes of the tumor-inducing plasmid. Maximum transformation frequency was obtained with exponentially growing plant cells, suggesting that rapid growth of plant cells is an essental factor for efficient transformation of higher plants.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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