Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1985 Oct;79(2):571–577. doi: 10.1104/pp.79.2.571

Nucleotide Sequence of the F1-ATPase α Subunit Gene from Maize Mitochondria 1

Carl J Braun 1, Charles S Levings III 1
PMCID: PMC1074929  PMID: 16664454

Abstract

The α subunit of the F1-ATPase complex of maize is a mitochondrial translational product, presumably encoded by the mitochondrial genome. Based on nucleotide and amino acid homology, we have identified a mitochondrial gene, designated atpα, that appears to code for the F1-ATPase α subunit of Zea mays. The atpα gene is present as a single copy in the maize. Texas cytoplasm and is actively transcribed. The maize α polypeptide has a predicted length of 508 amino acids and a molecular mass of 55,187 daltons. Amino acid homologies between the maize mitochondrial α subunit and the tobacco chloroplast CF1 and Escherichia coli α subunits are 54 and 51%, respectively. The origin of the atpα gene is discussed.

Full text

PDF
577

Images in this article

573Fig. 2
on p.573
576Fig. 6
on p.576

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boutry M., Briquet M., Goffeau A. The alpha subunit of a plant mitochondrial F1-ATPase is translated in mitochondria. J Biol Chem. 1983 Jul 25;258(14):8524–8526. [PubMed] [Google Scholar]
  2. Chao S., Sederoff R., Levings C. S., 3rd Nucleotide sequence and evolution of the 18S ribosomal RNA gene in maize mitochondria. Nucleic Acids Res. 1984 Aug 24;12(16):6629–6644. doi: 10.1093/nar/12.16.6629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dawson A. J., Jones V. P., Leaver C. J. The apocytochrome b gene in maize mitochondria does not contain introns and is preceded by a potential ribosome binding site. EMBO J. 1984 Sep;3(9):2107–2113. doi: 10.1002/j.1460-2075.1984.tb02098.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Deno H., Shinozaki K., Sugiura M. Nucleotide sequence of tobacco chloroplast gene for the alpha subunit of proton-translocating ATPase. Nucleic Acids Res. 1983 Apr 11;11(7):2185–2191. doi: 10.1093/nar/11.7.2185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dewey R. E., Schuster A. M., Levings C. S., Timothy D. H. Nucleotide sequence of F(0)-ATPase proteolipid (subunit 9) gene of maize mitochondria. Proc Natl Acad Sci U S A. 1985 Feb;82(4):1015–1019. doi: 10.1073/pnas.82.4.1015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fox T. D., Leaver C. J. The Zea mays mitochondrial gene coding cytochrome oxidase subunit II has an intervening sequence and does not contain TGA codons. Cell. 1981 Nov;26(3 Pt 1):315–323. doi: 10.1016/0092-8674(81)90200-2. [DOI] [PubMed] [Google Scholar]
  7. Gay N. J., Walker J. E. The atp operon: nucleotide sequence of the region encoding the alpha-subunit of Escherichia coli ATP-synthase. Nucleic Acids Res. 1981 May 11;9(9):2187–2194. doi: 10.1093/nar/9.9.2187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hack E., Leaver C. J. The alpha-subunit of the maize F(1)-ATPase is synthesised in the mitochondrion. EMBO J. 1983;2(10):1783–1789. doi: 10.1002/j.1460-2075.1983.tb01658.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hu N., Messing J. The making of strand-specific M13 probes. Gene. 1982 Mar;17(3):271–277. doi: 10.1016/0378-1119(82)90143-3. [DOI] [PubMed] [Google Scholar]
  10. Kozak M. Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles. Microbiol Rev. 1983 Mar;47(1):1–45. doi: 10.1128/mr.47.1.1-45.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lonsdale D. M., Hodge T. P., Howe C. J., Stern D. B. Maize mitochondrial DNA contains a sequence homologous to the ribulose-1,5-bisphosphate carboxylase large subunit gene of chloroplast DNA. Cell. 1983 Oct;34(3):1007–1014. doi: 10.1016/0092-8674(83)90558-5. [DOI] [PubMed] [Google Scholar]
  12. Norrander J., Kempe T., Messing J. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene. 1983 Dec;26(1):101–106. doi: 10.1016/0378-1119(83)90040-9. [DOI] [PubMed] [Google Scholar]
  13. Parks T. D., Dougherty W. G., Levings C. S., Timothy D. H. Identification of two methionine transfer RNA genes in the maize mitochondrial genome. Plant Physiol. 1984 Dec;76(4):1079–1082. doi: 10.1104/pp.76.4.1079. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pring D. R., Levings C. S. Heterogeneity of Maize Cytoplasmic Genomes among Male-Sterile Cytoplasms. Genetics. 1978 May;89(1):121–136. doi: 10.1093/genetics/89.1.121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  16. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Shine J., Dalgarno L. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342–1346. doi: 10.1073/pnas.71.4.1342. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Spitsberg V. L., Pfeiffer N. E., Partridge B., Wylie D. E., Schuster S. M. Isolation and Antigenic Characterization of Corn Mitochondrial F(1)-ATPase. Plant Physiol. 1985 Feb;77(2):339–345. doi: 10.1104/pp.77.2.339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stern D. B., Lonsdale D. M. Mitochondrial and chloroplast genomes of maize have a 12-kilobase DNA sequence in common. Nature. 1982 Oct 21;299(5885):698–702. doi: 10.1038/299698a0. [DOI] [PubMed] [Google Scholar]
  20. Stern D. B., Palmer J. D. Extensive and widespread homologies between mitochondrial DNA and chloroplast DNA in plants. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1946–1950. doi: 10.1073/pnas.81.7.1946. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES