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. 1986 Feb;80(2):310–314. doi: 10.1104/pp.80.2.310

Development of (1→3,1→4)-β-d-Glucan Endohydrolase Isoenzymes in Isolated Scutella and Aleurone Layers of Barley (Hordeum vulgare) 1

I Montgomery Stuart 1,2, Lin Loi 1, Geoffrey B Fincher 1
PMCID: PMC1075110  PMID: 16664619

Abstract

An immunological assay has been used to investigate the synthesis of (1→3,1→4)-β-glucanase (EC 3.2.1.73) isoenzymes from isolated barley aleurone layers and scutella. Enzyme release from both tissues is enhanced by 1 micromolar gibberellic acid and 10 millimolar Ca2+, although increases induced by gibberellic acid are observed only in the presence of Ca2+. Isoenzyme I is synthesized predominantly in the scutellum, while isoenzyme II is synthesized exclusively in the aleurone. A third, putative isoenzyme III has been detected in significant proportions in scutellar secretions and may also be secreted from aleurone layers. Both gibberellic acid and Ca2+ appear to preferentially enhance isoenzyme II secretion from the aleurone and isoenzyme III secretion from scutella. The patterns of isoenzyme secretion are suggestive of tissue-specific differences in expression of the genes which code for (1→3,1→4)-β-glucanase isoenzymes. Qualitatively similar results were obtained with barley cultivars harvested in Australia and North America.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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