Abstract
The effect of gibberellic acid and Ca2+ on the accumulation of α-amylase mRNAs in aleurone layers of barley (Hordeum vulgare L. cv Himalaya) was studied using cDNA clones containing sequences of mRNAs for the high and low isoelectric point (pI) α-amylases. There is no significant hybridization between the two α-amylase cDNA clones under the hybridization and washing conditions employed. These clones were therefore used to monitor levels of mRNAs for high and low pI α-amylases. It is shown that although the synthesis of the high pI α-amylase proteins depends on the presence of Ca2+ in the incubation medium, the accumulation of mRNA for this group occurs to the same degree in the presence or the absence of Ca2+. The accumulation of low pI α-amylase mRNA is also not affected by the presence or absence of Ca2+ in the incubation medium. These results establish gibberellic acid, not Ca2+, as the principal regulator of α-amylase mRNA accumulation in barley aleurone, while Ca2+ controls high pI α-amylase synthesis at a later step in the biosynthetic pathway.
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- Callis J., Ho T. H. Multiple molecular forms of the gibberellin-induced alpha-amylase from the aleurone layers of barley seeds. Arch Biochem Biophys. 1983 Jul 1;224(1):224–234. doi: 10.1016/0003-9861(83)90206-0. [DOI] [PubMed] [Google Scholar]
- Deikman J., Jones R. L. Control of alpha-amylase mRNA accumulation by gibberellic Acid and calcium in barley aleurone layers. Plant Physiol. 1985 May;78(1):192–198. doi: 10.1104/pp.78.1.192. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gerlach W. L., Bedbrook J. R. Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Res. 1979 Dec 11;7(7):1869–1885. doi: 10.1093/nar/7.7.1869. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Huang J. K., Swegle M., Dandekar A. M., Muthukrishnan S. Expression and regulation of alpha-amylase gene family in barley aleurones. J Mol Appl Genet. 1984;2(6):579–588. [PubMed] [Google Scholar]
- Jacobsen J. V., Higgins T. J. Characterization of the alpha-Amylases Synthesized by Aleurone Layers of Himalaya Barley in Response to Gibberellic Acid. Plant Physiol. 1982 Dec;70(6):1647–1653. doi: 10.1104/pp.70.6.1647. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jacobsen J. V., Scandalios J. G., Varner J. E. Multiple forms of amylase induced by gibberellic acid in isolated barley aleurone layers. Plant Physiol. 1970 Apr;45(4):367–371. doi: 10.1104/pp.45.4.367. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jones R. L., Carbonell J. Regulation of the synthesis of barley aleurone alpha-amylase by gibberellic Acid and calcium ions. Plant Physiol. 1984 Sep;76(1):213–218. doi: 10.1104/pp.76.1.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Muthukrishnan S., Chandra G. R., Maxwell E. S. Hormonal control of alpha-amylase gene expression in barley. Studies using a cloned CDNA probe. J Biol Chem. 1983 Feb 25;258(4):2370–2375. [PubMed] [Google Scholar]
- Muthukrishnan S., Gill B. S., Swegle M., Chandra G. R. Structural genes for alpha-amylases are located on barley chromosomes 1 and 6. J Biol Chem. 1984 Nov 25;259(22):13637–13639. [PubMed] [Google Scholar]
- Rogers J. C., Milliman C. Coordinate increase in major transcripts from the high pI alpha-amylase multigene family in barley aleurone cells stimulated with gibberellic acid. J Biol Chem. 1984 Oct 10;259(19):12234–12240. [PubMed] [Google Scholar]
- Rogers J. C., Milliman C. Isolation and sequence analysis of a barley alpha-amylase cDNA clone. J Biol Chem. 1983 Jul 10;258(13):8169–8174. [PubMed] [Google Scholar]
- Rogers J. C. Two barley alpha-amylase gene families are regulated differently in aleurone cells. J Biol Chem. 1985 Mar 25;260(6):3731–3738. [PubMed] [Google Scholar]