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. 1986 May;81(1):30–35. doi: 10.1104/pp.81.1.30

Accumulation of Chlorophyll, Chloroplastic Proteins, and Thylakoid Membranes during Reversion of Chromoplasts to Chloroplasts in Citrus sinensis Epicarp 1

Stephen P Mayfield 1,2,2, Albert Huff 1,2
PMCID: PMC1075277  PMID: 16664794

Abstract

In vitro culture of pericarp segments from fruit of Citrus sinensis (L.) Osbeck cv Valencia was used to determine the temporal sequence in development of chloroplasts from chromoplasts during regreening of the epicarp. Regreening of chromoplasts closely resembled greening of etioplasts, except that regreening proceeded much more slowly. Chlorophyll, the light-harvesting chlorophyll a/b binding protein of photosystem II, the chlorophyll a binding protein of reaction center P-700 of photosystem I, thylakoid membranes, and adenosine triphosphate synthetase were all detected at very low levels in degreened epicarp. All of these increased in parallel during regreening of the epicarp. Ribulose 1,5-bisphosphate carboxylase (RuBPCase) levels were high in degreened epicarp and declined for the first 10 days of culture before reaccumulating in the regreening segments. Light was necessary for the accumulation of all of the chloroplastic components. A lack of exogenous nitrogen did not prevent the accumulation of any chloroplastic component except Ru-BPCase, although accumulation of the other components was reduced. Sucrose at 150 millimolar in media lacking nitrogen markedly inhibited the accumulation of chlorophyll and light-harvesting chlorophyll a/b-protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson J. M. The molecular organization of chloroplast thylakoids. Biochim Biophys Acta. 1975 Aug 15;416(2):191–235. doi: 10.1016/0304-4173(75)90007-5. [DOI] [PubMed] [Google Scholar]
  2. Apel K., Kloppstech K. The plastid membranes of barley (Hordeum vulgare). Light-induced appearance of mRNA coding for the apoprotein of the light-harvesting chlorophyll a/b protein. Eur J Biochem. 1978 Apr 17;85(2):581–588. doi: 10.1111/j.1432-1033.1978.tb12273.x. [DOI] [PubMed] [Google Scholar]
  3. Argyroudi-Akoyunoglou J. H., Tsakiris S. Development of the cation-induced stacking capacity during the biogenesis of higher plant thylakoids. Arch Biochem Biophys. 1977 Nov;184(1):307–315. doi: 10.1016/0003-9861(77)90355-1. [DOI] [PubMed] [Google Scholar]
  4. Grebanier A. E., Steinback K. E., Bogorad L. Comparison of the Molecular Weights of Proteins Synthesized by Isolated Chloroplasts with Those Which Appear during Greening in Zea mays. Plant Physiol. 1979 Mar;63(3):436–439. doi: 10.1104/pp.63.3.436. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Huff A. Nutritional control of regreening and degreening in citrus peel segments. Plant Physiol. 1983 Oct;73(2):243–249. doi: 10.1104/pp.73.2.243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Huff A. Sugar regulation of plastid interconversions in epicarp of citrus fruit. Plant Physiol. 1984 Oct;76(2):307–312. doi: 10.1104/pp.76.2.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. KOSKI V. M., FRENCH C. S., SMITH J. H. C. The action spectrum for the transformation of protochlorophyll to chlorophyll a in normal and albino corn seedlings. Arch Biochem Biophys. 1951 Mar;31(1):1–17. doi: 10.1016/0003-9861(51)90178-6. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Moran R. Formulae for determination of chlorophyllous pigments extracted with n,n-dimethylformamide. Plant Physiol. 1982 Jun;69(6):1376–1381. doi: 10.1104/pp.69.6.1376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nelson T., Harpster M. H., Mayfield S. P., Taylor W. C. Light-regulated gene expression during maize leaf development. J Cell Biol. 1984 Feb;98(2):558–564. doi: 10.1083/jcb.98.2.558. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Spurr A. R. A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res. 1969 Jan;26(1):31–43. doi: 10.1016/s0022-5320(69)90033-1. [DOI] [PubMed] [Google Scholar]
  12. Tobin E. M. White Light Effects on the mRNA for the Light-Harvesting Chlorophyll a/b-Protein in Lemna gibba L. G-3. Plant Physiol. 1981 Jun;67(6):1078–1083. doi: 10.1104/pp.67.6.1078. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Vierling E., Alberte R. S. Regulation of synthesis of the photosystem I reaction center. J Cell Biol. 1983 Dec;97(6):1806–1814. doi: 10.1083/jcb.97.6.1806. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Walden R., Leaver C. J. Synthesis of Chloroplast Proteins during Germination and Early Development of Cucumber. Plant Physiol. 1981 Jun;67(6):1090–1096. doi: 10.1104/pp.67.6.1090. [DOI] [PMC free article] [PubMed] [Google Scholar]

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