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. 1986 Jun;81(2):516–521. doi: 10.1104/pp.81.2.516

Monoclonal Antibodies to Plant Growth Regulators

III. Zeatinriboside and Dihydrozeatinriboside

Joachim Eberle 1, Angelika Arnscheidt 1, Dieter Klix 1, Elmar W Weiler 1
PMCID: PMC1075368  PMID: 16664848

Abstract

Four high affinity monoclonal antibodies, which recognize two plant growth regulators from the cytokinin group, namely trans-zeatin riboside and dihydrozeatin riboside and their derivatives are reported. Six hybridomas were produced from three independent fusions of Balb/c spleen cells with P3-NS1-Ag 4-1 (abbreviated NS1) or X63-Ag 8.653 (X63) myeloma cells. The mice had been hyperimmunized with zeatin riboside-bovine serum albumin conjugate or dihydrozeatin riboside-bovine serum albumin conjugate for 3 months. The hybridomas secrete antibodies of the IgG 1 or IgG 2b subclass and allow the detection of femtomole amounts of the free cytokinins, their ribosides, and ribotides in plant extracts. The use of these monoclonals in radio- and enzyme-linked immunosorbent assay is also discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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