Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1986 Jun;81(2):714–716. doi: 10.1104/pp.81.2.714

Aphidicolin Inhibition of DNA Synthesis and Germination in Spores of Anemia phyllitidis L. Sw. 1

A Fechner 1, H Schraudolf 1
PMCID: PMC1075410  PMID: 16664889

Abstract

Aphidicolin inhibits DNA synthesis and nuclear division in spores of Anemia phyllitidis. In spite of blocked DNA replication, spores germinate under continuous dark conditions, if induced by addition of 5 × 10−5 grams per milliliter gibberellic acid. Differentiation of aphidicolin-treated prothallia indicate the existence of a prepattern in the dry spore which is realized independent of cell division during early events of spore germination.

Full text

PDF
715

Images in this article

715Fig. 2
on p.715
716Fig. 3
on p.716

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Geuskens M., Hardt N., Pedrali-Noy G., Spadari S. An autoradiographic demonstration of nuclear DNA replication by DNA polymerase alpha and of mitochondrial DNA synthesis by DNA polymerase gamma. Nucleic Acids Res. 1981 Apr 10;9(7):1599–1613. doi: 10.1093/nar/9.7.1599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Giulotto E., Mondello C. Aphidicolin does not inhibit the repair synthesis of mitotic chromosomes. Biochem Biophys Res Commun. 1981 Apr 30;99(4):1287–1294. doi: 10.1016/0006-291x(81)90759-2. [DOI] [PubMed] [Google Scholar]
  3. Hardt N., Pedrali-Noy G., Focher F., Spadari S. Aphidicolin does not inhibit DNA repair synthesis in ultraviolet-irradiated HeLa cells. A radioautographic study. Biochem J. 1981 Nov 1;199(2):453–455. doi: 10.1042/bj1990453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Huberman J. A. New views of the biochemistry of eucaryotic DNA replication revealed by aphidicolin, an unusual inhibitor of DNA polymerase alpha. Cell. 1981 Mar;23(3):647–648. doi: 10.1016/0092-8674(81)90426-8. [DOI] [PubMed] [Google Scholar]
  5. Raghavan V. Gibberellic acid-induced germination of spores of Anemia phyllitidis: autoradiographic study of the timing and regulation of nucleic acid and protein synthesis in relation to cell morphogenesis. J Cell Sci. 1977 Feb;23:85–100. doi: 10.1242/jcs.23.1.85. [DOI] [PubMed] [Google Scholar]
  6. Raghavan V. Synthesis of protein and RNA for initiation and growth of the protonema during germination of bracken fern spore. Exp Cell Res. 1971 Apr;65(2):401–407. doi: 10.1016/0014-4827(71)90019-x. [DOI] [PubMed] [Google Scholar]
  7. Sala F., Amileni A. R., Parisi B., Spadari S. A gamma-like DNA polymerase in spinach chloroplasts. Eur J Biochem. 1980 Nov;112(2):211–217. doi: 10.1111/j.1432-1033.1980.tb07196.x. [DOI] [PubMed] [Google Scholar]
  8. Sala F., Galli M. G., Levi M., Burroni D., Parisi B., Pedrali-Noy G., Spadari S. Functional roles of the plant alpha-like and gamma-like DNA polymerases. FEBS Lett. 1981 Feb 9;124(1):112–118. doi: 10.1016/0014-5793(81)80064-6. [DOI] [PubMed] [Google Scholar]
  9. Sala F., Magnien E., Galli M. G., Dalschaert X., Pedrali-Noy G., Spadari S. DNA repair synthesis in plant protoplasts is aphidicolin-resistant. FEBS Lett. 1982 Feb 22;138(2):213–217. doi: 10.1016/0014-5793(82)80444-4. [DOI] [PubMed] [Google Scholar]
  10. Sala F., Parisi B., Burroni D., Amileni A. R., Pedrali-Noy G., Spadari S. Specific and reversible inhibition by aphidicolin in the alpha-like DNA polymerase of plant cells. FEBS Lett. 1980 Aug 11;117(1):93–98. doi: 10.1016/0014-5793(80)80920-3. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES