Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1992 Dec;100(4):1968–1975. doi: 10.1104/pp.100.4.1968

Stimulation of Growth and Ion Uptake in Bean Leaves by Red and Blue Light 1

Dale E Blum 1, J Theo M Elzenga 1, Paul A Linnemeyer 1,2, Elizabeth Van Volkenburgh 1
PMCID: PMC1075892  PMID: 16653225

Abstract

Red and blue light both stimulate growth and ion accumulation in bean (Phaseolus vulgaris L.) leaves, and previous studies showed that the growth response is mediated by phytochrome and a blue-light receptor. Results of this study confirm that there is an additional photosynthetic contribution from the growing cells that supports ion uptake and growth. Disc expansion in the light was enhanced by exogenous K+ and Rb+, but was not specific for anions. Light increased K+ accumulation and the rate of 86Rb+ uptake by discs, over darkness, with no effect of light quality. The photosynthetic inhibitor, 3-(3,4-dichlorophenyl)-1,1-dimethylurea, inhibited light-driven 86Rb+ uptake by 75%. Light quality caused differences in short-term kinetics of growth and acidification of the leaf surface. At comparable fluence rates (50 μmol m−2 s−1), continuous exposure to blue light increased the growth rate 3-fold after a 2-min lag, whereas red light caused a smaller growth response after a lag of 12 min. In contrast, the acidification of the leaf surface normally associated with growth was stimulated 3-fold by red light but only slightly (1.3-fold) by blue light. This result shows that, in addition to acidification caused by red light, a second mechanism specifically stimulated by blue light is normally functioning in light-driven leaf growth.

Full text

PDF
1975

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chory J., Peto C. A., Ashbaugh M., Saganich R., Pratt L., Ausubel F. Different Roles for Phytochrome in Etiolated and Green Plants Deduced from Characterization of Arabidopsis thaliana Mutants. Plant Cell. 1989 Sep;1(9):867–880. doi: 10.1105/tpc.1.9.867. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cleland R. E., Buckley G., Nowbar S., Lew N. M., Stinemetz C., Evans M. L., Rayle D. L. The pH profile for acid-induced elongation of coleoptile and epicotyl sections is consistent with the acid-growth theory. Planta. 1991;186:70–74. [PubMed] [Google Scholar]
  3. Elzenga J. T., Prins H. B. Light-Induced Polar pH Changes in Leaves of Elodea canadensis: I. Effects of Carbon Concentration and Light Intensity. Plant Physiol. 1989 Sep;91(1):62–67. doi: 10.1104/pp.91.1.62. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kigel J., Cosgrove D. J. Photoinhibition of stem elongation by blue and red light: effects on hydraulic and cell wall properties. Plant Physiol. 1991;95:1049–1056. doi: 10.1104/pp.95.4.1049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Liscum E., Hangarter R. P. Arabidopsis Mutants Lacking Blue Light-Dependent Inhibition of Hypocotyl Elongation. Plant Cell. 1991 Jul;3(7):685–694. doi: 10.1105/tpc.3.7.685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. MACROBBIE E. A. THE NATURE OF THE COUPLING BETWEEN LIGHT ENERGY AND ACTIVE ION TRANSPORT IN NITELLA TRANSLUCENS. Biochim Biophys Acta. 1965 Jan 25;94:64–73. doi: 10.1016/0926-6585(65)90008-7. [DOI] [PubMed] [Google Scholar]
  7. Poffenroth M., Green D. B., Tallman G. Sugar Concentrations in Guard Cells of Vicia faba Illuminated with Red or Blue Light : Analysis by High Performance Liquid Chromatography. Plant Physiol. 1992 Apr;98(4):1460–1471. doi: 10.1104/pp.98.4.1460. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Rains D. W. Kinetics and Energetics of Light-enhanced Potassium Absorption by Corn Leaf Tissue. Plant Physiol. 1968 Mar;43(3):394–400. doi: 10.1104/pp.43.3.394. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Schmalstig J. G., Cosgrove D. J. Coupling of solute transport and cell expansion in pea stems. Plant Physiol. 1990;94:1625–1633. doi: 10.1104/pp.94.4.1625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Serrano E. E., Zeiger E., Hagiwara S. Red light stimulates an electrogenic proton pump in Vicia guard cell protoplasts. Proc Natl Acad Sci U S A. 1988 Jan;85(2):436–440. doi: 10.1073/pnas.85.2.436. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Spalding E. P., Cosgrove D. J. Large plasma-membrane depolarization precedes rapid blue-light-induced growth inhibition in cucumber. Planta. 1989;178:407–410. [PubMed] [Google Scholar]
  12. Van Volkenburgh E., Cleland R. E. Light-stimulated cell expansion in bean (Phaseolus vulgaris L.) leaves. I. Growth can occur without photosynthesis. Planta. 1990 Aug;182(1):72–76. doi: 10.1007/BF00239986. [DOI] [PubMed] [Google Scholar]
  13. Van Volkenburgh E., Cleland R. E., Watanabe M. Light-stimulated cell expansion in bean (Phaseolus vulgaris L.) leaves. II. Quantity and quality of light required. Planta. 1990 Aug;182(1):77–80. doi: 10.1007/BF00239987. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES