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. 1991 Feb;95(2):556–563. doi: 10.1104/pp.95.2.556

Direct Photolabeling with [32P]UDP-Glucose for Identification of a Subunit of Cotton Fiber Callose Synthase 1

Deborah P Delmer 1, Mazal Solomon 1, Stephen M Read 1,2
PMCID: PMC1077568  PMID: 16668019

Abstract

We have identified a 52 kilodalton polypeptide as being a likely candidate for the catalytic subunit of the UDP-glucose: (1→3)-β-glucan (callose) synthase of developing fibers of Gossypium hirsutum (cotton). Such a polypeptide migrates coincident with callose synthase during glycerol gradient centrifugation in the presence of EDTA, and can be directly photolabeled with the radioactive substrate, α-[32P]UDP-glucose. Interaction with the labeled probe requires Ca2+, a specific activator of callose synthase which is known to lower the Km of higher plant callose synthases for the substrate UDP-glucose. Using this probe and several other related ones, several other proteins which interact with UDP-glucose were also identified, but none satisfied all of the above criteria for being components of the callose synthase.

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Selected References

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