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. 1991 Mar;95(3):669–674. doi: 10.1104/pp.95.3.669

Anaerobic Stress Induces the Transcription and Translation of Sucrose Synthase in Rice

Bérénice Ricard 1, Jean Rivoal 1, Anne Spiteri 1, Alain Pradet 1
PMCID: PMC1077589  PMID: 16668037

Abstract

Sucrose synthase activity increased in 2-day-old rice (Oryza sativa) seedlings submitted to anaerobic stress. Likewise, both denaturing and native Western blot analysis detected a rise in the cellular concentration of sucrose synthase protein. Significantly higher steady-state levels of sucrose synthase mRNA, as determined by Northern blots and by the ability of total RNA to direct in vitro synthesis of sucrose synthase, were also induced by anaerobic treatment. Analysis of run-on transcripts showed increased transcription of sucrose synthase genes as early as 60 minutes after initiation of anaerobic stress. Together, these results indicate that sucrose synthase is a typical anaerobic protein in rice.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aspart L., Got A., Delseny M., Mocquot B., Pradet A. Adaptation of ribonucleic Acid metabolism to anoxia in rice embryos. Plant Physiol. 1983 May;72(1):115–121. doi: 10.1104/pp.72.1.115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beach L. R., Spencer D., Randall P. J., Higgins T. J. Transcriptional and post-transcriptional regulation of storage protein gene expression in sulfur-deficient pea seeds. Nucleic Acids Res. 1985 Feb 11;13(3):999–1013. doi: 10.1093/nar/13.3.999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Echt C. S., Chourey P. S. A Comparison of Two Sucrose Synthetase Isozymes from Normal and shrunken-1 Maize. Plant Physiol. 1985 Oct;79(2):530–536. doi: 10.1104/pp.79.2.530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hake S., Kelley P. M., Taylor W. C., Freeling M. Coordinate induction of alcohol dehydrogenase 1, aldolase, and other anaerobic RNAs in maize. J Biol Chem. 1985 Apr 25;260(8):5050–5054. [PubMed] [Google Scholar]
  5. Kodrzycki R., Boston R. S., Larkins B. A. The opaque-2 mutation of maize differentially reduces zein gene transcription. Plant Cell. 1989 Jan;1(1):105–114. doi: 10.1105/tpc.1.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kopczynski C. C., Scandalios J. G. Cat-2 gene expression. Developmental control of translatable CAT-2 mRNA levels in maize scutellum. Mol Gen Genet. 1986 Apr;203(1):185–188. doi: 10.1007/BF00330401. [DOI] [PubMed] [Google Scholar]
  7. Luthe D. S., Quatrano R. S. Transcription in Isolated Wheat Nuclei: II. CHARACTERIZATION OF RNA SYNTHESIZED IN VITRO. Plant Physiol. 1980 Feb;65(2):309–313. doi: 10.1104/pp.65.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. McCarty D. R., Shaw J. R., Hannah L. C. The cloning, genetic mapping, and expression of the constitutive sucrose synthase locus of maize. Proc Natl Acad Sci U S A. 1986 Dec;83(23):9099–9103. doi: 10.1073/pnas.83.23.9099. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McElfresh K. C., Chourey P. S. Anaerobiosis induces transcription but not translation of sucrose synthase in maize. Plant Physiol. 1988 Jun;87(2):542–546. doi: 10.1104/pp.87.2.542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mocquot B., Prat C., Mouches C., Pradet A. Effect of anoxia on energy charge and protein synthesis in rice embryo. Plant Physiol. 1981 Sep;68(3):636–640. doi: 10.1104/pp.68.3.636. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mocquot B., Ricard B., Pradet A. Rice embryos can express heat-shock genes under anoxia. Biochimie. 1987 Jun-Jul;69(6-7):677–681. doi: 10.1016/0300-9084(87)90188-x. [DOI] [PubMed] [Google Scholar]
  12. Sachs M. M., Freeling M., Okimoto R. The anaerobic proteins of maize. Cell. 1980 Jul;20(3):761–767. doi: 10.1016/0092-8674(80)90322-0. [DOI] [PubMed] [Google Scholar]
  13. Springer B., Werr W., Starlinger P., Bennett D. C., Zokolica M., Freeling M. The Shrunken gene on chromosome 9 of Zea mays L is expressed in various plant tissues and encodes an anaerobic protein. Mol Gen Genet. 1986 Dec;205(3):461–468. doi: 10.1007/BF00338083. [DOI] [PubMed] [Google Scholar]
  14. Werr W., Frommer W. B., Maas C., Starlinger P. Structure of the sucrose synthase gene on chromosome 9 of Zea mays L. EMBO J. 1985 Jun;4(6):1373–1380. doi: 10.1002/j.1460-2075.1985.tb03789.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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