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. 1992 May;99(1):134–139. doi: 10.1104/pp.99.1.134

Glycine Uptake into Barley Mesophyll Vacuoles Is Regulated but Not Energized by ATP 1

Joern Goerlach 1,2, Indra Willms-Hoff 1,3
PMCID: PMC1080417  PMID: 16668840

Abstract

[U-14C]glycine uptake into barley (Hordeum vulgare cv Hasso) vacuoles was investigated. Glycine (2 millimolar) transport was stimulated two- to fourfold by NaATP. Stimulation was saturable with respect to ATP (1 millimolar) and linear up to 20 millimolar glycine. Stimulation by NaATP was suppressed by Mg2+ in equimolar amounts. Neither MgATP nor Mg-inorganic pyrophosphate had any effect on basal transport rate. Thus, the proton motive force can be excluded as the driving force. Uncouplers (valinomycine/carbonylcyanide-m-chlorophenylhydrazone) inhibited the basal rate up to 30% but had no influence on NaATP-stimulated uptake. Vanadate had no effect on either basal or NaATP-stimulated uptake. Nonhydrolyzable ATP analogs (adenylyl(β, γ-methylen)-diphosphate or adenylyl-imidodiphosphate) stimulated comparable to NaATP. Other nucleotides (UTP, ADP) had no effect. Some evidence exists that other amino acids (arginine, alanine, isoleucine, phenylalanine) are transported to a certain extent by a similar mechanism. The results indicate a high capacity channel-like translocator that is regulated but not energized by ATP.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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