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. 1992 Jun;99(2):508–514. doi: 10.1104/pp.99.2.508

Effect of Inorganic Cations and Metabolic Inhibitors on Putrescine Transport in Roots of Intact Maize Seedlings

Joseph M DiTomaso 1,2, Jonathan J Hart 1,2, Dean L Linscott 1,2, Leon V Kochian 1,2
PMCID: PMC1080492  PMID: 16668915

Abstract

The specificity and regulation of putrescine transport was investigated in roots of intact maize (Zea mays L.) seedlings. In concentration-dependent transport studies, the kinetics for putrescine uptake could be resolved into a single saturable component that was noncompetitively inhibited by increasing concentrations of Ca2+ (50 micromolar to 5 millimolar). Similarly, other polyvalent cations, including Mg2+ (1.8 millimolar) and La3+ (200 micromolar), almost completely abolished the saturable component for putrescine uptake. This suggests that putrescine does not share a common transport system with other divalent or polyvalent inorganic cations. Further characterization of the putrescine transport system indicated that 0.3 millimolar N-ethyl-maleimide had no effect on putrescine uptake, and 2 millimolar p-chloromercuribenzene sulfonic acid only partially inhibited transport of the diamine (39% inhibition). Metabolic inhibitors, including carbonylcyanide-m-chlorphenylhydrazone (20 micromolar) and KCN (0.5 millimolar), also partially inhibited the saturable component for putrescine uptake (Vmax reduced 48-60%). Increasing the time of exposure to carbonylcyanide-m-chlorphenylhydrazone from 30 minutes to 2 hours did not significantly increase the inhibition of putrescine uptake. Electrophysiological evidence indicates that the inhibitory effect on putrescine uptake by these inhibitors is correlated to a depolarization of the membrane potential, suggesting that the driving force for putrescine uptake is the transmembrane electrical potential across the plasmalemma.

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Selected References

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