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. 1992 Jul;99(3):1084–1089. doi: 10.1104/pp.99.3.1084

Antioxidants and Manganese Deficiency in Needles of Norway Spruce (Picea abies L.) Trees 1

Andrea Polle 1,2, Krisanu Chakrabarti 1,2,2, Sila Chakrabarti 1,2,2, Friederike Seifert 1,2, Peter Schramel 1,2, Heinz Rennenberg 1,2
PMCID: PMC1080587  PMID: 16668974

Abstract

Chlorotic and green needles from Norway spruce (Picea abies L.) trees were sampled in the Calcareous Bavarian Alps in winter. The needles were used for analysis of the mineral and pigment contents, the levels of antioxidants (ascorbate, glutathione), and the activities of protective enzymes (superoxide dismutase, catalase, ascorbate peroxidase, monodehydroascorbate radical reductase, dehydroascorbate reductase, glutathione reductase). In addition, the activities of two respiratory enzymes (glucose-6-phosphate dehydrogenase, NAD-malate dehydrogenase), which might provide the NADPH necessary for functioning of the antioxidative system, were determined. We found that chlorotic needles were severely manganese deficient (3 to 6 micrograms Mn per gram dry weight as compared with up to 190 micrograms Mn per gram dry weight in green needles) but had a similar dry weight to fresh weight ratio, had a similar protein content, and showed no evidence for enhanced lipid peroxidation as compared with green needles. In chlorotic needles, the level of total ascorbate and the activities of superoxide dismutase, monodehydroascorbate radical reductase, NAD-malate dehydrogenase, and glucose-6-phosphate dehydrogenase were significantly increased, whereas the levels of ascorbate peroxidase, dehydroascorbate reductase, glutathione reductase, and glutathione were not affected. The ratio of ascorbate to dehydroascorbate was similar in both green and chlorotic needles. These results suggest that in spruce needles monodehydroascorbate radical reductase is the key enzyme involved in maintaining ascorbate in its reduced state. The reductant necessary for this process may have been supplied at the expense of photosynthate.

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Selected References

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  1. Berger E., Foster P., Laskowski L., Lu G., Oren D., Pardo D. Managing the medical record on a concurrent basis: facts and possibilities. Part 2. J Am Med Rec Assoc. 1988 Jun;59(6):25–37. [PubMed] [Google Scholar]
  2. Cakmak I., Marschner H. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves. Plant Physiol. 1992 Apr;98(4):1222–1227. doi: 10.1104/pp.98.4.1222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Esterbauer H., Grill D. Seasonal Variation of Glutathione and Glutathione Reductase in Needles of Picea abies. Plant Physiol. 1978 Jan;61(1):119–121. doi: 10.1104/pp.61.1.119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. McCord J. M., Fridovich I. The utility of superoxide dismutase in studying free radical reactions. I. Radicals generated by the interaction of sulfite, dimethyl sulfoxide, and oxygen. J Biol Chem. 1969 Nov 25;244(22):6056–6063. [PubMed] [Google Scholar]
  5. Peever T. L., Higgins V. J. Electrolyte Leakage, Lipoxygenase, and Lipid Peroxidation Induced in Tomato Leaf Tissue by Specific and Nonspecific Elicitors from Cladosporium fulvum. Plant Physiol. 1989 Jul;90(3):867–875. doi: 10.1104/pp.90.3.867. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Polle A., Chakrabarti K., Schürmann W., Renneberg H. Composition and Properties of Hydrogen Peroxide Decomposing Systems in Extracellular and Total Extracts from Needles of Norway Spruce (Picea abies L., Karst.). Plant Physiol. 1990 Sep;94(1):312–319. doi: 10.1104/pp.94.1.312. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Polle A., Krings B., Rennenberg H. Superoxide Dismutase Activity in Needles of Norwegian Spruce Trees (Picea abies L.). Plant Physiol. 1989 Aug;90(4):1310–1315. doi: 10.1104/pp.90.4.1310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Senser M., Kloos M., Lütz C. Influence of soil substrate and ozone plus acid mist on the pigment content and composition of needles from young Norway spruce trees. Environ Pollut. 1990;64(3-4):295–312. doi: 10.1016/0269-7491(90)90052-e. [DOI] [PubMed] [Google Scholar]
  9. Wise R. R., Naylor A. W. Chilling-enhanced photooxidation : evidence for the role of singlet oxygen and superoxide in the breakdown of pigments and endogenous antioxidants. Plant Physiol. 1987 Feb;83(2):278–282. doi: 10.1104/pp.83.2.278. [DOI] [PMC free article] [PubMed] [Google Scholar]

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