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. 1992 Aug;99(4):1362–1369. doi: 10.1104/pp.99.4.1362

Association of 70-Kilodalton Heat-Shock Cognate Proteins with Acclimation to Cold 1

Lisa G Neven 1,2,3, Dale W Haskell 1,2,3, Charles L Guy 1,2,3, Nancy Denslow 1,2,3, Paul A Klein 1,2,3, Linda G Green 1,2,3, Allison Silverman 1,2,3
PMCID: PMC1080633  PMID: 16669045

Abstract

Exposure of young spinach seedlings (Spinacia oleracea L. cv Bloomsdale) to 5°C leads to an increase in the synthesis of several 79-kilodalton proteins that are present in leaf tissue grown at 20°C. Protein sequence analyses and immunological cross-reactivity indicate that this group of proteins belongs to the 70-kilodalton heat-shock family. Steady-state transcript levels and protein synthesis are increased two- to threefold within 1 day, but immunoblot analyses suggest that the steady-state concentration of this protein group in leaf tissue only gradually accumulates at low temperature. It is proposed that the increased synthesis of several members of the 70-kilodalton heat-shock family could result from an influence of low temperature on protein folding and/or assembly processes.

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