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. 1992 Aug;99(4):1388–1392. doi: 10.1104/pp.99.4.1388

The Antiozonant Ethylenediurea Does Not Act via Superoxide Dismutase Induction in Bean 1

Lynne H Pitcher 1,2, Eileen Brennan 1,2, Barbara A Zilinskas 1,2
PMCID: PMC1080637  PMID: 16669049

Abstract

It has been proposed that the mode of action of ethylenediurea, a very effective antiozonant, is via an increase in the antioxidant enzyme superoxide dismutase (EH Lee, JH Bennett [1982] Plant Physiol 69: 1444-1449). Data presented here refute that hypothesis. No ethylenediurea-associated increases in Cu/Zn-superoxide dismutase or Mn-superoxide dismutase activity, nor in steady-state Cu/Zn-superoxide dismutase protein levels, were found in soluble extracts of bean (Phaseolus vulgaris L. cv Bush Blue Lake 290) leaves. However, the cytosolic Cu/Zn-superoxide dismutase increased as a result of ozone fumigation and subsequent injury. Also noted was a developmentally related difference between chloroplastic and cytosolic Cu/Zn-superoxide dismutase, the latter declining during maturation of the leaf.

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Selected References

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