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. 1992 Aug;99(4):1551–1555. doi: 10.1104/pp.99.4.1551

Metabolism of 2′-Carboxyarabinitol in Leaves 1

Brandon d Moore 1, Jeffrey R Seemann 1
PMCID: PMC1080662  PMID: 16669073

Abstract

Results presented here indicate that 2′-carboxyarabinitol (CA) is the in vivo precursor and product of 2′-carboxyarabinitol 1-phosphate (CA1P) metabolism in leaves. When [2-14C]CA was fed in the light to leaves of five species known to be highly active in CA1P metabolism (Phaseolus vulgaris, Lycopersicon esculentum, Helianthus annuus, Petunia hybrida, and Beta vulgaris), [14C]CA1P was formed in the dark. Reillumination of a Phaseolus leaf caused this [14C]CA1P to be rapidly metabolized to [14C]CA (t½ = 1 min). The epimer 2′-carboxyribitol could not substitute for CA in the dark synthesis of CA1P, and CA in the anionic form was a better substrate than CA in the lactone form. In leaves of Phaseolus vulgaris, the active CA pool size used in the dark synthesis of CA1P is between about 70 and 110 nanomoles per milligram of chlorophyll. The photosynthetic electron transport inhibitor diuron did not affect the dark synthesis of [14C]CA1P, but did greatly reduce the rate of its subsequent light degradation (t½ = approximately 10 min). Dark synthesis of [14C]CA1P was inhibited by dithiothreitol and NaF. From the present data, we suggest that CA1P and CA participate in a metabolic substrate cycle in vivo.

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Selected References

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  1. Beck E., Scheibe R., Reiner J. An Assessment of the Rubisco Inhibitor: 2-Carboxyarabinitol-1-Phosphate and d-Hamamelonic Acid 2-Phosphate Are Identical Compounds. Plant Physiol. 1989 May;90(1):13–16. doi: 10.1104/pp.90.1.13. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berry J. A., Lorimer G. H., Pierce J., Seemann J. R., Meek J., Freas S. Isolation, identification, and synthesis of 2-carboxyarabinitol 1-phosphate, a diurnal regulator of ribulose-bisphosphate carboxylase activity. Proc Natl Acad Sci U S A. 1987 Feb;84(3):734–738. doi: 10.1073/pnas.84.3.734. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gutteridge S., Reddy G. S., Lorimer G. The synthesis and purification of 2'-carboxy-D-arabinitol 1-phosphate, a natural inhibitor of ribulose 1,5-bisphosphate carboxylase, investigated by 31P n.m.r. Biochem J. 1989 Jun 15;260(3):711–716. doi: 10.1042/bj2600711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Holbrook G. P., Bowes G., Salvucci M. E. Degradation of 2-carboxyarabinitol 1-phosphate by a specific chloroplast phosphatase. Plant Physiol. 1989 Jun;90(2):673–678. doi: 10.1104/pp.90.2.673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Holbrook G. P., Galasinski S. C., Salvucci M. E. Regulation of 2-carboxyarabinitol 1-phosphatase. Plant Physiol. 1991 Nov;97(3):894–899. doi: 10.1104/pp.97.3.894. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kobza J., Seemann J. R. Light-dependent kinetics of 2-carboxyarabinitol 1-phosphate metabolism and ribulose-1,5-bisphosphate carboxylase activity in vivo. Plant Physiol. 1989 Jan;89(1):174–179. doi: 10.1104/pp.89.1.174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Moore B. D., Kobza J., Seemann J. R. Measurement of 2-carboxyarabinitol 1-phosphate in plant leaves by isotope dilution. Plant Physiol. 1991 May;96(1):208–213. doi: 10.1104/pp.96.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Moore B. D., Sharkey T. D., Kobza J., Seemann J. R. Identification and levels of 2'-carboxyarabinitol in leaves. Plant Physiol. 1992 Aug;99(4):1546–1550. doi: 10.1104/pp.99.4.1546. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Pierce J., Tolbert N. E., Barker R. Interaction of ribulosebisphosphate carboxylase/oxygenase with transition-state analogues. Biochemistry. 1980 Mar 4;19(5):934–942. doi: 10.1021/bi00546a018. [DOI] [PubMed] [Google Scholar]
  10. Redgwell R. J. Fractionation of plant extracts using ion-exchange Sephadex. Anal Biochem. 1980 Sep 1;107(1):44–50. doi: 10.1016/0003-2697(80)90489-3. [DOI] [PubMed] [Google Scholar]
  11. Salvucci M. E., Holbrook G. P. Purification and Properties of 2-Carboxy-d-Arabinitol 1-Phosphatase. Plant Physiol. 1989 Jun;90(2):679–685. doi: 10.1104/pp.90.2.679. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schürmann P. Separation of phosphate esters and algal extracts by thin-layer electrophoresis and chromatography. J Chromatogr. 1969 Feb 25;39(4):507–509. [PubMed] [Google Scholar]
  13. Seemann J. R., Berry J. A., Freas S. M., Krump M. A. Regulation of ribulose bisphosphate carboxylase activity in vivo by a light-modulated inhibitor of catalysis. Proc Natl Acad Sci U S A. 1985 Dec;82(23):8024–8028. doi: 10.1073/pnas.82.23.8024. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Servaites J. C. Inhibition of ribulose 1,5-bisphosphate carboxylase/oxygenase by 2-carboxyarabinitol-1-phosphate. Plant Physiol. 1990 Apr;92(4):867–870. doi: 10.1104/pp.92.4.867. [DOI] [PMC free article] [PubMed] [Google Scholar]

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