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. 1992 Aug;99(4):1686–1688. doi: 10.1104/pp.99.4.1686

Localized Patch Clamping of Plasma Membrane of a Polarized Plant Cell 1

Laser Microsurgery of the Fucus spiralis Rhizoid Cell Wall

Alison R Taylor 1, Colin Brownlee 1
PMCID: PMC1080682  PMID: 16669092

Abstract

We used an ultraviolet laser to rupture a small region of cell wall of a polarized Fucus spiralis rhizoid cell and gained localized access to the plasma membrane at the growing apex. Careful control of cell turgor enabled a small portion of plasma membrane-bound cytoplasm to be exposed. Gigaohm seals allowing single-channel recordings were obtained with a high success rate using this method with conventional patch clamp techniques.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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