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. 1991 May;96(1):319–323. doi: 10.1104/pp.96.1.319

Protein Synthesis in Isolated Mitochondria of Rice (Oryza sativa L.) Seedlings 1

Hwa Dai 1,2,3,4, Yih-Shan Lo 1,2,3,4, Chiung-Yuan Wu 1,2,3,4, Chia-Lin Tsou 1,2,3,4, Gon-Shi Hsu 1,2,3,4, Chyr-Guan Chern 1,2,3,4, Manfred Ruddat 1,2,3,4, Kwen-Sheng Chiang 1,2,3,4
PMCID: PMC1080754  PMID: 16668173

Abstract

For studies of in organello mitochondrial protein synthesis in rice, Oryza sativa L., conventional surface-sterilization procedures were demonstrated to be ineffective. Because of the over-whelmingly efficient [35S]methionine utilization by contaminating bacteria, even “essentially bacteria-free” rice mitochondria were shown to be unsuitable for the study of in organello protein synthesis. We developed a procedure to obtain a bacteria-free preparation of rice mitochondria. Such mitochondria favored a membrane-dependent ATP-generating system over an external ATP-generating system as the energy supplement for in organello protein synthesis. Two distinct classes of [35S]methionine-labeled, cycloheximide-insensitive products were detected: an electrophoretically unresolved population and a set of some 22 to 27 discrete polypeptide species, each with a characteristic electrophoretic mobility and relative abundance.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Forde B. G., Leaver C. J. Nuclear and cytoplasmic genes controlling synthesis of variant mitochondrial polypeptides in male-sterile maize. Proc Natl Acad Sci U S A. 1980 Jan;77(1):418–422. doi: 10.1073/pnas.77.1.418. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Forde B. G., Oliver R. J., Leaver C. J. In Vitro Study of Mitochondrial Protein Synthesis during Mitochondrial Biogenesis in Excised Plant Storage Tissue. Plant Physiol. 1979 Jan;63(1):67–73. doi: 10.1104/pp.63.1.67. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Forde B. G., Oliver R. J., Leaver C. J. Variation in mitochondrial translation products associated with male-sterile cytoplasms in maize. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3841–3845. doi: 10.1073/pnas.75.8.3841. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  5. Leaver C. J., Hack E., Forde B. G. Protein synthesis by isolated plant mitochondria. Methods Enzymol. 1983;97:476–484. doi: 10.1016/0076-6879(83)97156-2. [DOI] [PubMed] [Google Scholar]
  6. Nebiolo C. M., White E. M. Corn mitochondrial protein synthesis in response to heat shock. Plant Physiol. 1985 Dec;79(4):1129–1132. doi: 10.1104/pp.79.4.1129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Nieto-Sotelo J., Ho T. H. Absence of heat shock protein synthesis in isolated mitochondria and plastids from maize. J Biol Chem. 1987 Sep 5;262(25):12288–12292. [PubMed] [Google Scholar]
  8. Sinibaldi R. M., Turpen T. A heat shock protein is encoded within mitochondria of higher plants. J Biol Chem. 1985 Dec 15;260(29):15382–15385. [PubMed] [Google Scholar]

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