Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1991 Sep;97(1):288–297. doi: 10.1104/pp.97.1.288

Activity and Accumulation of Cell Division-Promoting Phenolics in Tobacco Tissue Cultures 1

Rita A Teutonico 1,2,2, Matthew W Dudley 1,2, John D Orr 1,2,3, David G Lynn 1,2, Andrew N Binns 1,2
PMCID: PMC1080996  PMID: 16668384

Abstract

Dehydrodiconiferyl alcohol glucosides (DCGs) are derivatives of the phenylpropanoid pathway that have been isolated from Catharansus roseus L. (Vinca rosea) crown gall tumors. Fractions containing purified DCGs have been shown previously to promote the growth of cytokinin-requiring tissues of tobacco in the absence of exogenous cytokinins. In this study, we utilized synthetic DCG isomers to confirm the cell division-promoting activity of DCG isomers A and B and show that they neither promote shoot meristem initiation on Nicotiana tabacum L., cv Havana 425, leaf explants nor induce betacyanin synthesis in amaranth seedlings. Analysis of cultured tobacco pith tissue demonstrated that DCG accumulation was stimulated by cytokinin treatment and correlated with cytokinin-induced cell division. Thus, the accumulation of metabolites that could replace cytokinin in cell division bioassays is stimulated by cytokinins. These data support the model that DCGs are a component of a cytokinin-mediated regulatory circuit controlling cell division.

Full text

PDF
297

Images in this article

292Figure 2
on p.292

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Binns A. N., Chen R. H., Wood H. N., Lynn D. G. Cell division promoting activity of naturally occurring dehydrodiconiferyl glucosides: do cell wall components control cell division? Proc Natl Acad Sci U S A. 1987 Feb;84(4):980–984. doi: 10.1073/pnas.84.4.980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Binns A., Meins F. Habituation of tobacco pith cells for factors promoting cell division is heritable and potentially reversible. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2660–2662. doi: 10.1073/pnas.70.9.2660. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kuboi T., Yamada Y. Regulation of the enzyme activities related to lignin synthesis in cell aggregates of tobacco cell culture. Biochim Biophys Acta. 1978 Aug 17;542(2):181–190. doi: 10.1016/0304-4165(78)90014-4. [DOI] [PubMed] [Google Scholar]
  4. Lynn D. G., Chen R. H., Manning K. S., Wood H. N. The structural characterization of endogenous factors from Vinca rosea crown gall tumors that promote cell division of tobacco cells. Proc Natl Acad Sci U S A. 1987 Feb;84(3):615–619. doi: 10.1073/pnas.84.3.615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Miller C. O. Ribosyl-trans-Zeatin, A Major Cytokinin Produced by Crown Gall Tumor Tissue. Proc Natl Acad Sci U S A. 1974 Feb;71(2):334–338. doi: 10.1073/pnas.71.2.334. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Murray A. W., Kirschner M. W. Dominoes and clocks: the union of two views of the cell cycle. Science. 1989 Nov 3;246(4930):614–621. doi: 10.1126/science.2683077. [DOI] [PubMed] [Google Scholar]
  7. SKOOG F., MILLER C. O. Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symp Soc Exp Biol. 1957;11:118–130. [PubMed] [Google Scholar]
  8. Shinshi H., Mohnen D., Meins F. Regulation of a plant pathogenesis-related enzyme: Inhibition of chitinase and chitinase mRNA accumulation in cultured tobacco tissues by auxin and cytokinin. Proc Natl Acad Sci U S A. 1987 Jan;84(1):89–93. doi: 10.1073/pnas.84.1.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Wood H. N., Braun A. C., Brandes H., Kende H. Studies on the distribution and properties of a new class of cell division--promoting substances from higher plant species. Proc Natl Acad Sci U S A. 1969 Feb;62(2):349–356. doi: 10.1073/pnas.62.2.349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Wood H. N., Lin M. C., Braun A. C. The inhibition of plant and animal adenosine 3':5'-cyclic monophosphate phosphodiesterases by a cell-division-promoting substance from tissues of higher plant species. Proc Natl Acad Sci U S A. 1972 Feb;69(2):403–406. doi: 10.1073/pnas.69.2.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Wood H. N., Rennekamp M. E., Bowen D. V., Field F. H., Braun A. C. A comparative study of cytokinesins I and II and zeatin riboside: a reply to Carlos Miller. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4140–4143. doi: 10.1073/pnas.71.10.4140. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES