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. 1991 Sep;97(1):420–425. doi: 10.1104/pp.97.1.420

Changes in Photorespiratory Enzyme Activity in Response to Limiting CO2 in Chlamydomonas reinhardtii 1

Laura Fredrick Marek 1, Martin H Spalding 1
PMCID: PMC1081015  PMID: 16668403

Abstract

The activity of two photorespiratory enzymes, phosphoglycolate phosphatase (PGPase) and glycolate dehydrogenase (glycolate DH), changes when CO2-enriched wild-type (WT) Chlamydomonas reinhardtii cells are transferred to air levels of CO2. Adaptation to air levels of CO2 by Chlamydomonas involves induction of a CO2-concentrating mechanism (CCM) which increases the internal inorganic carbon concentration and suppresses oxygenase activity of ribulose-1,5-bisphosphate carboxylase/oxygenase. PGPase in cell extracts shows a transient increase in activity that reaches a maximum 3 to 5 hours after transfer and then declines to the original level within 48 hours. The decline in PGPase activity begins at about the time that physiological evidence indicates the CCM is approaching maximal activity. Glycolate DH activity in 24 hour air-adapted WT cells is double that seen in CO2-enriched cells. Unlike WT, the high-CO2-requiring mutant, cia-5, does not respond to limiting CO2 conditions: it does not induce any known aspects of the CCM and it does not show changes in PGPase or glycolate DH activities. Other known mutants of the CCM show patterns of PGPase and glycolate DH activity after transfer to limiting CO2 which are different from WT and cia-5 but which are consistent with changes in activity being initiated by the same factor that induces the CCM, although secondary regulation must also be involved.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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