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. 1991 Oct;97(2):693–698. doi: 10.1104/pp.97.2.693

Elicitor-Inducible 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase Activity Is Required for Sesquiterpene Accumulation in Tobacco Cell Suspension Cultures 1

Joseph Chappell 1, Carol VonLanken 1, Urs Vögeli 1,2
PMCID: PMC1081062  PMID: 16668454

Abstract

Addition of cell wall fragments from Phytophthora species or cellulase from Trichoderma viride, but not pectolyase from Aspergillus japonicus, to tobacco (Nicotiana tabacum) cell suspension cultures induced the accumulation of the extracellular sesquiterpenoid capsidiol. Pulse-labeling experiments with [14C]acetate and [3H]mevalonate suggested that enzymatic steps preceding mevalonate were limiting capsidiol biosynthesis in the pectolyase-treated cell cultures. Treatment of the cell cultures with either Phytophthora cell wall fragments or cellulase induced 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) and sesquiterpene cyclase activities, enzymes of the sesquiterpene biosynthetic pathway, and phenylalanine ammonia lyase activity, an enzyme of the general phenylpropanoid pathway. Pectolyase treatment induced sesquiterpene cyclase and phenylalanine ammonia lyase activities, but not HMGR activity. These results corroborate the importance of inducible HMGR enzyme activity for sesquiterpene accumulation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ayers A. R., Ebel J., Valent B., Albersheim P. Host-Pathogen Interactions: X. Fractionation and Biological Activity of an Elicitor Isolated from the Mycelial Walls of Phytophthora megasperma var. sojae. Plant Physiol. 1976 May;57(5):760–765. doi: 10.1104/pp.57.5.760. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bruce R. J., West C. A. Elicitation of Casbene Synthetase Activity in Castor Bean : THE ROLE OF PECTIC FRAGMENTS OF THE PLANT CELL WALL IN ELICITATION BY A FUNGAL ENDOPOLYGALACTURONASE. Plant Physiol. 1982 May;69(5):1181–1188. doi: 10.1104/pp.69.5.1181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chappell J., Nable R. Induction of sesquiterpenoid biosynthesis in tobacco cell suspension cultures by fungal elicitor. Plant Physiol. 1987 Oct;85(2):469–473. doi: 10.1104/pp.85.2.469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Davis K. R., Darvill A. G., Albersheim P., Dell A. Host-Pathogen Interactions : XXIX. Oligogalacturonides Released from Sodium Polypectate by Endopolygalacturonic Acid Lyase Are Elicitors of Phytoalexins in Soybean. Plant Physiol. 1986 Feb;80(2):568–577. doi: 10.1104/pp.80.2.568. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Davis K. R., Hahlbrock K. Induction of defense responses in cultured parsley cells by plant cell wall fragments. Plant Physiol. 1987 Aug;84(4):1286–1290. doi: 10.1104/pp.84.4.1286. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Davis K. R., Lyon G. D., Darvill A. G., Albersheim P. Host-Pathogen Interactions : XXV. Endopolygalacturonic Acid Lyase from Erwinia carotovora Elicits Phytoalexin Accumulation by Releasing Plant Cell Wall Fragments. Plant Physiol. 1984 Jan;74(1):52–60. doi: 10.1104/pp.74.1.52. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hahn M. G., Darvill A. G., Albersheim P. Host-Pathogen Interactions : XIX. THE ENDOGENOUS ELICITOR, A FRAGMENT OF A PLANT CELL WALL POLYSACCHARIDE THAT ELICITS PHYTOALEXIN ACCUMULATION IN SOYBEANS. Plant Physiol. 1981 Nov;68(5):1161–1169. doi: 10.1104/pp.68.5.1161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lee S. C., West C. A. Polygalacturonase from Rhizopus stolonifer, an Elicitor of Casbene Synthetase Activity in Castor Bean (Ricinus communis L.) Seedlings. Plant Physiol. 1981 Apr;67(4):633–639. doi: 10.1104/pp.67.4.633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nothnagel E. A., McNeil M., Albersheim P., Dell A. Host-Pathogen Interactions : XXII. A Galacturonic Acid Oligosaccharide from Plant Cell Walls Elicits Phytoalexins. Plant Physiol. 1983 Apr;71(4):916–926. doi: 10.1104/pp.71.4.916. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sharp J. K., Valent B., Albersheim P. Purification and partial characterization of a beta-glucan fragment that elicits phytoalexin accumulation in soybean. J Biol Chem. 1984 Sep 25;259(18):11312–11320. [PubMed] [Google Scholar]
  11. Stermer B. A., Bostock R. M. Involvement of 3-hydroxy-3-methylglutaryl coenzyme a reductase in the regulation of sesquiterpenoid phytoalexin synthesis in potato. Plant Physiol. 1987 Jun;84(2):404–408. doi: 10.1104/pp.84.2.404. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Vögeli U., Chappell J. Induction of sesquiterpene cyclase and suppression of squalene synthetase activities in plant cell cultures treated with fungal elicitor. Plant Physiol. 1988 Dec;88(4):1291–1296. doi: 10.1104/pp.88.4.1291. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Walker-Simmons M., Jin D., West C. A., Hadwiger L., Ryan C. A. Comparison of proteinase inhibitor-inducing activities and phytoalexin elicitor activities of a pure fungal endopolygalacturonase, pectic fragments, and chitosans. Plant Physiol. 1984 Nov;76(3):833–836. doi: 10.1104/pp.76.3.833. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Zimmermann S., Hahlbrock K. Light-induced changes of enzyme activities in parsley cell suspension cultures. Purification and some properties of phenylalanine ammonia-lyase (E.C.4.3.1.5). Arch Biochem Biophys. 1975 Jan;166(1):54–62. doi: 10.1016/0003-9861(75)90364-1. [DOI] [PubMed] [Google Scholar]

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