Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1966 Jun;41(6):913–918. doi: 10.1104/pp.41.6.913

Effects of Light Intensity on Photosynthetic Carboxylative Phase Enzymes and Chlorophyll Synthesis in Greening Leaves of Hordeum vulgare L

R C Huffaker 1, R L Obendorf 1, C J Keller 1, G E Kleinkopf 1
PMCID: PMC1086451  PMID: 16656355

Abstract

The effects of various light intensities on in vivo increases in activities of phosphoriboisomerase, phosphoribulokinase and ribulose-1, 5-diP carboxylase and on synthesis of chlorophyll were studied in greening leaves of Hordeum vulgare L.

Each enzyme was already present in dark-grown plants, but further increases in activities required both a light treatment of the intact plant and a favorable temperature. The amount of enzymatic activity and chlorophyll developed was governed by light intensity.

Measured activities of phosphoriboisomerase and ribulose 1,5-diP carboxylase were highly correlated with synthesis of chlorophyll at all intensities studied. Measured activity of phosphoribulokinase was correlated with synthesis of chlorophyll only at saturating or near saturating light intensities. At decreasing light intensities the response curves of this enzyme differed from those of chlorophyll and of phosphoriboisomerase and ribulose-1, 5-diP carboxylase. A lag period of phosphoribulokinase increased with decreasing light intensity. After the lag period a rapid rate of increase occurred which did not level off during 48 hours of illumination. Thus, a different control mechanism may be operative in inducing increased activity of this enzyme.

Full text

PDF
915

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ASHWELL G., HICKMAN J. Enzymatic formation of xylulose 5-phosphate from ribose 5-phosphate in spleen. J Biol Chem. 1957 May;226(1):65–76. [PubMed] [Google Scholar]
  2. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DISCHE Z., BORENFREUND E. A new spectrophotometric method for the detection and determination of keto sugars and trioses. J Biol Chem. 1951 Oct;192(2):583–587. [PubMed] [Google Scholar]
  4. HALL D. O., HUFFAKER R. C., SHANNON L. M., WALLACE A. Influence of light on dark carboxylation reactions in etiolated barley leaves. Biochim Biophys Acta. 1959 Oct;35:540–542. doi: 10.1016/0006-3002(59)90406-8. [DOI] [PubMed] [Google Scholar]
  5. HURWITZ J., WEISSBACH A., HORECKER B. L., SMYRNIOTIS P. Z. Spinach phosphoribulokinase. J Biol Chem. 1956 Feb;218(2):769–783. [PubMed] [Google Scholar]
  6. Hudock G. A., McLeod G. C., Moravkova-Kiely J., Levine R. P. The Relation of Oxygen Evolution to Chlorophyll and Protein Synthesis in a Mutant Strain of Chlamydomonas reinhardi. Plant Physiol. 1964 Nov;39(6):898–903. doi: 10.1104/pp.39.6.898. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Margulies M. M. Effect of Chloramphenicol on Light-Dependent Synthesis of Proteins and Enzymes of Leaves and Chloroplasts of Phaseolus vulgaris. Plant Physiol. 1964 Jul;39(4):579–585. doi: 10.1104/pp.39.4.579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. TROWN P. W. AN IMPROVED METHOD FOR THE ISOLATION OF CARBOXYDISMUTASE. PROBABLE IDENTITY WITH FRACTION I PROTEIN AND THE PROTEIN MOIETY OF PROTOCHLOROPHYLL HOLOCHROME. Biochemistry. 1965 May;4:908–918. doi: 10.1021/bi00881a018. [DOI] [PubMed] [Google Scholar]
  10. WEISSBACH A., HORECKER B. L., HURWITZ J. The enzymatic formation of phosphoglyceric acid from ribulose diphosphate and carbon dioxide. J Biol Chem. 1956 Feb;218(2):795–810. [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES