Skip to main content
Plant Physiology logoLink to Plant Physiology
. 1966 Jun;41(6):976–982. doi: 10.1104/pp.41.6.976

Association of D-RNA with Polyribosomes in the Soybean Root 1,2

C Y Lin 1, Joe L Key 1, C E Bracker 1
PMCID: PMC1086462  PMID: 16656365

Abstract

Ribosomes from soybean root tips were shown to consist of about 75% polyribosomes based on size distribution on sucrose gradients. Electronmicrographs showed the presence of ribosome clusters in normal preparations and only monomers and dimers in ribonuclease-treated preparations. The polyribosome preparations, but not single ribosomes, showed good in vitro amino acid incorporating activity.

D-RNA was associated with the ribosome preparations, and preferentially in the polyribosome structure. It is suggested that at least a part of the soybean D-RNA represents a class of messenger RNA.

Full text

PDF

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAYLEY S. T. PHYSICAL STUDIES ON RIBOSOMES FROM PEA SEEDLINGS. J Mol Biol. 1964 Feb;8:231–238. doi: 10.1016/s0022-2836(64)80132-7. [DOI] [PubMed] [Google Scholar]
  2. CLARK M. F., MATTHEWS R. E., RALPH R. K. RIBOSOMES AND POLYRIBOSOMES IN BRASSICA PEKINENSIS. Biochim Biophys Acta. 1964 Oct 16;91:289–304. doi: 10.1016/0926-6550(64)90253-1. [DOI] [PubMed] [Google Scholar]
  3. DURE L., WATERS L. LONG-LIVED MESSENGER RNA: EVIDENCE FROM COTTON SEED GERMINATION. Science. 1965 Jan 22;147(3656):410–412. doi: 10.1126/science.147.3656.410. [DOI] [PubMed] [Google Scholar]
  4. GIERER A. Function of aggregated reticulocyte ribosomes in protein synthesis. J Mol Biol. 1963 Feb;6:148–157. doi: 10.1016/s0022-2836(63)80131-x. [DOI] [PubMed] [Google Scholar]
  5. GILBERT W. Polypeptide synthesis in Escherichia coli. I. Ribosomes and the active complex. J Mol Biol. 1963 May;6:374–388. doi: 10.1016/s0022-2836(63)80050-9. [DOI] [PubMed] [Google Scholar]
  6. HARDESTY B., MILLER R., SCHWEET R. POLYRIBOSOME BREAKDOWN AND HEMOGLOBIN SYNTHESIS. Proc Natl Acad Sci U S A. 1963 Nov;50:924–931. doi: 10.1073/pnas.50.5.924. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. INGLE J., KEY J. L., HOLM R. E. DEMONSTRATION AND CHARACTERIZATION OF A DNA-LIKE RNA IN EXCISED PLANT TISSUE. J Mol Biol. 1965 Apr;11:730–746. doi: 10.1016/s0022-2836(65)80031-6. [DOI] [PubMed] [Google Scholar]
  8. Ingle J., Key J. L. A comparative evaluation of the synthesis of DNA-like RNA in excised and intact plant tissues. Plant Physiol. 1965 Nov;40(6):1212–1219. doi: 10.1104/pp.40.6.1212. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Key J. L., Ingle J. REQUIREMENT FOR THE SYNTHESIS OF DNA-LIKE RNA FOR GROWTH OF EXCISED PLANT TISSUE. Proc Natl Acad Sci U S A. 1964 Dec;52(6):1382–1388. doi: 10.1073/pnas.52.6.1382. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LOENING U. E. THE SYNTHESIS OF MESSENGER AND RIBOSOMAL RNA IN PEA SEEDLINGS AS DETECTED BY ELECTROPHORESIS. Proc R Soc Lond B Biol Sci. 1965 Mar 16;162:121–136. doi: 10.1098/rspb.1965.0028. [DOI] [PubMed] [Google Scholar]
  11. MARCUS A., FEELEY J. PROTEIN SYNTHESIS IN IMBIBED SEEDS. II. POLYSOME FORMATION DURING IMBIBITION. J Biol Chem. 1965 Apr;240:1675–1680. [PubMed] [Google Scholar]
  12. MARCUS L., BRETTHAUER R., HALVORSON H., BOCK R. POLYSOMES FROM YEAST: DISTRIBUTION OF MESSENGER RNA AND CAPACITY TO SUPPORT PROTEIN SYNTHESIS IN VITRO. Science. 1965 Feb 5;147(3658):615–617. doi: 10.1126/science.147.3658.615. [DOI] [PubMed] [Google Scholar]
  13. Marks P. A., Burka E. R., Schlessinger D. PROTEIN SYNTHESIS IN ERYTHROID CELLS, I. RETICULOCYTE RIBOSOMES ACTIVE IN STIMULATING AMINO ACID INCORPORATION. Proc Natl Acad Sci U S A. 1962 Dec;48(12):2163–2171. doi: 10.1073/pnas.48.12.2163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. RISEBROUGH R. W., TISSIERES A., WATSON J. D. Messenger-RNA attachment to active ribosomes. Proc Natl Acad Sci U S A. 1962 Mar 15;48:430–436. doi: 10.1073/pnas.48.3.430. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Warner J. R., Rich A., Hall C. E. Electron Microscope Studies of Ribosomal Clusters Synthesizing Hemoglobin. Science. 1962 Dec 28;138(3548):1399–1403. doi: 10.1126/science.138.3548.1399. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES