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. 1966 Jun;41(6):976–982. doi: 10.1104/pp.41.6.976

Association of D-RNA with Polyribosomes in the Soybean Root 1,2

C Y Lin 1, Joe L Key 1, C E Bracker 1
PMCID: PMC1086462  PMID: 16656365

Abstract

Ribosomes from soybean root tips were shown to consist of about 75% polyribosomes based on size distribution on sucrose gradients. Electronmicrographs showed the presence of ribosome clusters in normal preparations and only monomers and dimers in ribonuclease-treated preparations. The polyribosome preparations, but not single ribosomes, showed good in vitro amino acid incorporating activity.

D-RNA was associated with the ribosome preparations, and preferentially in the polyribosome structure. It is suggested that at least a part of the soybean D-RNA represents a class of messenger RNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAYLEY S. T. PHYSICAL STUDIES ON RIBOSOMES FROM PEA SEEDLINGS. J Mol Biol. 1964 Feb;8:231–238. doi: 10.1016/s0022-2836(64)80132-7. [DOI] [PubMed] [Google Scholar]
  2. CLARK M. F., MATTHEWS R. E., RALPH R. K. RIBOSOMES AND POLYRIBOSOMES IN BRASSICA PEKINENSIS. Biochim Biophys Acta. 1964 Oct 16;91:289–304. doi: 10.1016/0926-6550(64)90253-1. [DOI] [PubMed] [Google Scholar]
  3. DURE L., WATERS L. LONG-LIVED MESSENGER RNA: EVIDENCE FROM COTTON SEED GERMINATION. Science. 1965 Jan 22;147(3656):410–412. doi: 10.1126/science.147.3656.410. [DOI] [PubMed] [Google Scholar]
  4. GIERER A. Function of aggregated reticulocyte ribosomes in protein synthesis. J Mol Biol. 1963 Feb;6:148–157. doi: 10.1016/s0022-2836(63)80131-x. [DOI] [PubMed] [Google Scholar]
  5. GILBERT W. Polypeptide synthesis in Escherichia coli. I. Ribosomes and the active complex. J Mol Biol. 1963 May;6:374–388. doi: 10.1016/s0022-2836(63)80050-9. [DOI] [PubMed] [Google Scholar]
  6. HARDESTY B., MILLER R., SCHWEET R. POLYRIBOSOME BREAKDOWN AND HEMOGLOBIN SYNTHESIS. Proc Natl Acad Sci U S A. 1963 Nov;50:924–931. doi: 10.1073/pnas.50.5.924. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. INGLE J., KEY J. L., HOLM R. E. DEMONSTRATION AND CHARACTERIZATION OF A DNA-LIKE RNA IN EXCISED PLANT TISSUE. J Mol Biol. 1965 Apr;11:730–746. doi: 10.1016/s0022-2836(65)80031-6. [DOI] [PubMed] [Google Scholar]
  8. Ingle J., Key J. L. A comparative evaluation of the synthesis of DNA-like RNA in excised and intact plant tissues. Plant Physiol. 1965 Nov;40(6):1212–1219. doi: 10.1104/pp.40.6.1212. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Key J. L., Ingle J. REQUIREMENT FOR THE SYNTHESIS OF DNA-LIKE RNA FOR GROWTH OF EXCISED PLANT TISSUE. Proc Natl Acad Sci U S A. 1964 Dec;52(6):1382–1388. doi: 10.1073/pnas.52.6.1382. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LOENING U. E. THE SYNTHESIS OF MESSENGER AND RIBOSOMAL RNA IN PEA SEEDLINGS AS DETECTED BY ELECTROPHORESIS. Proc R Soc Lond B Biol Sci. 1965 Mar 16;162:121–136. doi: 10.1098/rspb.1965.0028. [DOI] [PubMed] [Google Scholar]
  11. MARCUS A., FEELEY J. PROTEIN SYNTHESIS IN IMBIBED SEEDS. II. POLYSOME FORMATION DURING IMBIBITION. J Biol Chem. 1965 Apr;240:1675–1680. [PubMed] [Google Scholar]
  12. MARCUS L., BRETTHAUER R., HALVORSON H., BOCK R. POLYSOMES FROM YEAST: DISTRIBUTION OF MESSENGER RNA AND CAPACITY TO SUPPORT PROTEIN SYNTHESIS IN VITRO. Science. 1965 Feb 5;147(3658):615–617. doi: 10.1126/science.147.3658.615. [DOI] [PubMed] [Google Scholar]
  13. Marks P. A., Burka E. R., Schlessinger D. PROTEIN SYNTHESIS IN ERYTHROID CELLS, I. RETICULOCYTE RIBOSOMES ACTIVE IN STIMULATING AMINO ACID INCORPORATION. Proc Natl Acad Sci U S A. 1962 Dec;48(12):2163–2171. doi: 10.1073/pnas.48.12.2163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. RISEBROUGH R. W., TISSIERES A., WATSON J. D. Messenger-RNA attachment to active ribosomes. Proc Natl Acad Sci U S A. 1962 Mar 15;48:430–436. doi: 10.1073/pnas.48.3.430. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Warner J. R., Rich A., Hall C. E. Electron Microscope Studies of Ribosomal Clusters Synthesizing Hemoglobin. Science. 1962 Dec 28;138(3548):1399–1403. doi: 10.1126/science.138.3548.1399. [DOI] [PubMed] [Google Scholar]

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