Abstract
Phytochrome has been isolated from the green alga Mesotaenium and the liverwort Sphaerocarpos. The Mesotaenium pigment had absorption peaks at 649 and 710 nm for the PR and PFR forms, respectively. Corresponding difference spectrum maxima for the Sphaerocarpos pigment were at 655 and 720 nm. While the absorption maxima differ, the reversibility and efficiency with which red and far-red light transform the Mesotaenium pigment are very similar to that reported for phytochrome isolated from etiolated seedlings of higher plants. Methods are described which allow efficient separation of phytochrome from highly pigmented light-grown material.
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Selected References
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- Gassner E. B. On the Pigment Absorbing at 750 mmu Occurring in Some Blue-Green Algae. Plant Physiol. 1962 Sep;37(5):637–639. doi: 10.1104/pp.37.5.637. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lane H. C., Siegelman H. W., Butler W. L., Firer E. M. Detection of Phytochrome in Green Plants. Plant Physiol. 1963 Jul;38(4):414–416. doi: 10.1104/pp.38.4.414. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SAGER R., GRANICK S. Nutritional studies with Chlamydomonas reinhardi. Ann N Y Acad Sci. 1953 Oct 14;56(5):831–838. doi: 10.1111/j.1749-6632.1953.tb30261.x. [DOI] [PubMed] [Google Scholar]
- SIEGELMAN H. W., FIRER E. M. PURIFICATION OF PHYTOCHROME FROM OAT SEEDLINGS. Biochemistry. 1964 Mar;3:418–423. doi: 10.1021/bi00891a019. [DOI] [PubMed] [Google Scholar]
- Siegelman H. W., Turner B. C., Hendricks S. B. The chromophore of phytochrome. Plant Physiol. 1966 Oct;41(8):1289–1292. doi: 10.1104/pp.41.8.1289. [DOI] [PMC free article] [PubMed] [Google Scholar]