Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1967 Aug;42(8):1035–1041. doi: 10.1104/pp.42.8.1035

Biogenesis of Mitochondria in Germinating Peanut Cotyledons II. Changes in Cytochromes and Mitochondrial DNA 1

R W Breidenbach 1,2,2, Paul Castelfranco 1,2, R S Criddle 1,2
PMCID: PMC1086670  PMID: 16656611

Abstract

Biogenesis of mitochondria occurs in the germinating cotyledons of peanuts. This process was demonstrated by measuring both constitutive and enzymatic properties of mitochondria as a function of germination time. Direct counting by phase contrast microscopy of sucrose density gradient preparations showed that the number of mitochondria increased markedly during germination. DNA with a buoyant density distinct from the major cellular DNA was associated with these mitochondrial preparations. During germination the amount of this DNA in mitochondrial pellets increased. This increase closely paralleled the increase in number of mitochondria.

Succinoxidase and succinic dehydrogenase increased during germination. Both activities were confined to the mitochondrial fraction. The rate of increase of succinoxidase activity was significantly greater than the rate of increase of succinic dehydrogenase and both increased at least initially at a greater rate than the amount of mitochondrial DNA or numbers of mitochondria.

The amounts of cytochromes present in mitochondrial preparations were measured spectrophotometrically. All of the cytochromes increased in amount during germination. The rate of increase of cytochrome a — a3 very close to the rate of increase in succinoxidase activity.

Full text

PDF
1037

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AKAZAWA T., BEEVERS H. Mitochondria in the endosperm of the germinating castor bean; a developmental study. Biochem J. 1957 Sep;67(1):115–118. doi: 10.1042/bj0670115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cherry J. H. Nucleic Acid, Mitochondria, & Enzyme Changes in Cotyledons of Peanut Seeds during Germination. Plant Physiol. 1963 Jul;38(4):440–446. doi: 10.1104/pp.38.4.440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Corneo G., Moore C., Sanadi D. R., Grossman L. I., Marmur J. Mitochondrial DNA in yeast and some mammalian species. Science. 1966 Feb 11;151(3711):687–689. doi: 10.1126/science.151.3711.687. [DOI] [PubMed] [Google Scholar]
  5. Hiatt A. J. Preparation & some properties of soluble succinic dehydrogenase from higher plants. Plant Physiol. 1961 Sep;36(5):552–557. doi: 10.1104/pp.36.5.552. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LINNANE A. W., VITOLS E., NOWLAND P. G. Studies on the origin of yeast mitochondria. J Cell Biol. 1962 May;13:345–350. doi: 10.1083/jcb.13.2.345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. LUCK D. J., REICH E. DNA IN MITOCHONDRIA OF NEUROSPORA CRASSA. Proc Natl Acad Sci U S A. 1964 Oct;52:931–938. doi: 10.1073/pnas.52.4.931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rebeiz C., Castelfranco P. An Extra-Mitochondrial Enzyme System from Peanuts Catalyzing the beta-Oxidation of Fatty Acids. Plant Physiol. 1964 Nov;39(6):932–938. doi: 10.1104/pp.39.6.932. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. SCHATZ G. THE ISOLATION OF POSSIBLE MITOCHONDRIAL PRECURSOR STRUCTURES FROM AEROBICALLY GROWN BAKER'S YEAST. Biochem Biophys Res Commun. 1963 Aug 20;12:448–451. doi: 10.1016/0006-291x(63)90313-9. [DOI] [PubMed] [Google Scholar]
  11. SCHILDKRAUT C. L., MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol. 1962 Jun;4:430–443. doi: 10.1016/s0022-2836(62)80100-4. [DOI] [PubMed] [Google Scholar]
  12. Suyama Y., Bonner W. D. DNA from plant mitochondria. Plant Physiol. 1966 Mar;41(3):383–388. doi: 10.1104/pp.41.3.383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. WALLACE P. G., LINNANE A. W. OXYGEN-INDUCED SYNTHESIS OF YEAST MITOCHONDRIA. Nature. 1964 Mar 21;201:1191–1194. doi: 10.1038/2011191a0. [DOI] [PubMed] [Google Scholar]
  14. Young J. L., Huang R. C., Vanecko S., Marks J. D., Varner J. E. Conditions Affecting Enzyme Synthesis in Cotyledons of Germinating Seeds. Plant Physiol. 1960 May;35(3):288–292. doi: 10.1104/pp.35.3.288. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES