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. 1968 Apr;43(4):578–582. doi: 10.1104/pp.43.4.578

Plant Leaf and Stem Proteins. I. Extraction and Electrophoretic Separation of the Basic, Water-Soluble Fraction 1,2,3

B H McCown 1, G E Beck 1, T C Hall 1
PMCID: PMC1086890  PMID: 16656809

Abstract

Simplified but highly reproducible extraction and electrophoretic procedures have been developed for plant stem and leaf proteins using recent chemical and technical advances. The method is applied to the separation of the basic, water-soluble proteins found in stem and leaf tissues of 3 Dianthus clones. While most of the highly reproducible protein bands appear in all 3 selections, and many are also common to both leaf and stem tissue, others are characteristic for the variety or tissue.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blanquet R., Lenhoff H. M. A disulfide-linked collagenous protein of nematocyst capsules. Science. 1966 Oct 7;154(3745):152–153. doi: 10.1126/science.154.3745.152. [DOI] [PubMed] [Google Scholar]
  2. CLELAND W. W. DITHIOTHREITOL, A NEW PROTECTIVE REAGENT FOR SH GROUPS. Biochemistry. 1964 Apr;3:480–482. doi: 10.1021/bi00892a002. [DOI] [PubMed] [Google Scholar]
  3. Good N. E., Winget G. D., Winter W., Connolly T. N., Izawa S., Singh R. M. Hydrogen ion buffers for biological research. Biochemistry. 1966 Feb;5(2):467–477. doi: 10.1021/bi00866a011. [DOI] [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. Mourad N., Parks R. E., Jr Erythrocytic nucleoside diphosphokinase. 3. Studies with free and phosphorylated enzyme and evidence for an essential thiol group. J Biol Chem. 1966 Aug 25;241(16):3838–3844. [PubMed] [Google Scholar]
  6. STEWARD F. C., LYNDON R. F., BARBER J. T. ACRYLAMIDE GEL ELECTROPHORESIS OF SOLUBLE PLANT PROTEINS: A STUDY ON PEA SEEDLINGS IN RELATION TO DEVELOPMENT. Am J Bot. 1965 Feb;52:155–164. [PubMed] [Google Scholar]

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