Abstract
The chloroplast ribosomal-RNAs (1.1 × 106 and 0.56 × 106 mol wt) are synthesized in the normal ratio of 2:1. The non-ribosomal distribution observed after extraction and fractionation results from the lability of the 1.1 × 106 component, and a correction for this breakdown can be applied in certain cases. Newly synthesized 1.1 × 106 RNA is more stable than the older accumulated 1.1 × 106 RNA. Accumulation of the chloroplast RNA during growth of radish cotyledons occurs at a later time than the accumulation of cytoplasmic RNA, and its turnover is much less than that of the cytoplasmic ribosomal-RNA.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BRAWERMAN G., EISENSTADT J. M. TEMPLATE AND RIBOSOMAL RIBONUCLEIC ACIDS ASSOCIATED WITH THE CHLOROPLASTS AND THE CYTOPLASM OF EUGLENA GRACILIS. J Mol Biol. 1964 Dec;10:403–411. doi: 10.1016/s0022-2836(64)80061-9. [DOI] [PubMed] [Google Scholar]
- Dyer T. A., Leech R. M. Chloroplast and cytoplasmic low-molecular-weight ribonucleic acid components of the leaf of Vicia faba L. Biochem J. 1968 Feb;106(3):689–698. doi: 10.1042/bj1060689. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ingle J., Key J. L. A re-evaluation of the fractionation of high molecular weight RNA by MAK chromatography. Biochem Biophys Res Commun. 1968 Mar 27;30(6):711–716. doi: 10.1016/0006-291x(68)90571-8. [DOI] [PubMed] [Google Scholar]
- Loening U. E., Ingle J. Diversity of RNA components in green plant tissues. Nature. 1967 Jul 22;215(5099):363–367. doi: 10.1038/215363a0. [DOI] [PubMed] [Google Scholar]
- Stutz E., Noll H. Characterization of cytoplasmic and chloroplast polysomes in plants: evidence for three classes of ribosomal RNA in nature. Proc Natl Acad Sci U S A. 1967 Mar;57(3):774–781. doi: 10.1073/pnas.57.3.774. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zeldin M. H., Schiff J. A. RNA metabolism during light-induced chloroplast development in euglena. Plant Physiol. 1967 Jul;42(7):922–932. doi: 10.1104/pp.42.7.922. [DOI] [PMC free article] [PubMed] [Google Scholar]