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. 1968 Nov;43(11):1859–1865. doi: 10.1104/pp.43.11.1859

Synthesis of Chymotrypsin Inhibitor I Protein in Potato Leaflets Induced by Detachment 1

C A Ryan 1,2
PMCID: PMC1087090  PMID: 5699150

Abstract

Chymotrypsin inhibitor I is a protein that can be induced to accumulate in potato leaflets within hr in the light when leaflets are detached from intact plants and supplied with water. This increase in inhibitor I is not accompanied by an increase in all proteins in the detached leaflets. The accumulation is polar, proceeding from the base of the leaflets toward the tip. Low inhibitor I levels can be effectively maintained in detached leaflets by supplying them either water in the dark or solutions of indole acetic acid or 2,4-dichlorophenoxy acetic acid in the light. Inhibitor I from leaflets is not identical to inhibitor I isolated from potato tubers as determined by immunochemical analyses.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Key J. L. Ribonucleic Acid and Protein Synthesis as Essential Processes for Cell Elongation. Plant Physiol. 1964 May;39(3):365–370. doi: 10.1104/pp.39.3.365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  3. Ockerse R., Siegel B. Z., Galston A. W. Hormone-induced repression of a peroxidase isozyme in plant tissue. Science. 1966 Jan 28;151(3709):452–453. doi: 10.1126/science.151.3709.452. [DOI] [PubMed] [Google Scholar]
  4. RYAN C. A., BALLS A. K. An inhibitor of chymotrypsin from Solanum tuberosm and its behavior toward trypsin. Proc Natl Acad Sci U S A. 1962 Oct 15;48:1839–1844. doi: 10.1073/pnas.48.10.1839. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ryan C. A. Chymotrypsin inhibitor I from potatoes: reactivity with mammalian, plant, bacterial, and fungal proteinases. Biochemistry. 1966 May;5(5):1592–1596. doi: 10.1021/bi00869a020. [DOI] [PubMed] [Google Scholar]
  6. Ryan C. A., Huisman O. C., Van Denburgh R. W. Transitory Aspects of a Single Protein in Tissues of Solanum tuberosum and Its Coincidence With the Establishment of New Growth. Plant Physiol. 1968 Apr;43(4):589–596. doi: 10.1104/pp.43.4.589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ryan C. A. Quantitative determination of soluble cellular proteins by radial diffusion in agar gels containing antibodies. Anal Biochem. 1967 Jun;19(3):434–440. doi: 10.1016/0003-2697(67)90233-3. [DOI] [PubMed] [Google Scholar]

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