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. 1978 Jul;62(1):97–100. doi: 10.1104/pp.62.1.97

Biosynthesis of Ribulose-1,5-Bisphosphate Carboxylase in Spinach Leaf Protoplasts 1

Mikio Nishimura 1, Takashi Akazawa 1
PMCID: PMC1092063  PMID: 16660478

Abstract

Spinach leaf (Spinacia oleracea L. var. Kyoho) protoplasts sustain protein-synthesizing activity as measured by the incorporation of [14C]-leucine into the protein fraction both in the light and in the dark. By the immunoprecipitation of ribulose-1,5-bisphosphate (RuP2) carboxylase with rabbit antibody raised against the purified spinach enzyme preparation, it was found that approximately 7% of the total radiocarbon incorporated into the protein fraction in the light was in the carboxylase molecules. However, there was no measurable net increase observed in the content of the enzyme protein in the experimental conditions employed. It was found that both chloramphenicol and cycloheximide inhibited the incorporation of [14C]leucine into RuP2 carboxylase and its constituent subunits, as measured by the immunoprecipitation of the enzyme molecule and its subunits, A and B.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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