Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1978 Sep;62(3):470–472. doi: 10.1104/pp.62.3.470

Confounding of Alternate Respiration by Lipoxygenase Activity 1

David J Parrish 1,2, A Carl Leopold 1,3
PMCID: PMC1092149  PMID: 16660540

Abstract

The initial burst of respiratory activity (Qo2) of imbibing soybean (Glycine max [L.] Merr. var. Wayne) seed tissue is cyanide-insensitive, and sensitive to salicylhydroxamate: presumptive evidence for the presence of alternate respiration. The initial O2 consumption is also highly sensitive to propyl gallate. Soybean lipoxygenase exhibits similar characteristics of insensitivity to cyanide and sensitivity to salicylhydroxamate and to propyl gallate. The initial burst of respiration is enhanced by the addition of linoleic acid, a lipoxygenase substrate. These results indicate that the conventional tests for alternate respiration in plant tissues can be confounded by lipoxygenase; they also suggest that propyl gallate can be used to assess the possible participation of lipoxygenase in the O2 uptake by plant tissues.

Full text

PDF
471

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bahr J. T., Bonner W. D., Jr Cyanide-insensitive respiration. I. The steady states of skunk cabbage spadix and bean hypocotyl mitochondria. J Biol Chem. 1973 May 25;248(10):3441–3445. [PubMed] [Google Scholar]
  2. Chan H. W. Soya-bean lipoxygenase: an iron-containing dioxygenase. Biochim Biophys Acta. 1973 Nov 15;327(1):32–35. doi: 10.1016/0005-2744(73)90100-9. [DOI] [PubMed] [Google Scholar]
  3. Christopher J., Pistorius E., Axelrod B. Isolation of an isozyme of soybean lipoxygenase. Biochim Biophys Acta. 1970 Jan 14;198(1):12–19. doi: 10.1016/0005-2744(70)90028-8. [DOI] [PubMed] [Google Scholar]
  4. Parrish D. J., Leopold A. C. Transient changes during soybean imbibition. Plant Physiol. 1977 Jun;59(6):1111–1115. doi: 10.1104/pp.59.6.1111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Schonbaum G. R., Bonner W. D., Jr, Storey B. T., Bahr J. T. Specific inhibition of the cyanide-insensitive respiratory pathway in plant mitochondria by hydroxamic acids. Plant Physiol. 1971 Jan;47(1):124–128. doi: 10.1104/pp.47.1.124. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Solomos T., Laties G. G. The mechanism of ethylene and cyanide action in triggering the rise in respiration in potato tubers. Plant Physiol. 1975 Jan;55(1):73–78. doi: 10.1104/pp.55.1.73. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Surrey K. Spectrophotometric Method for Determination of Lipoxidase Activity. Plant Physiol. 1964 Jan;39(1):65–70. doi: 10.1104/pp.39.1.65. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Waring A. J., Laties G. G. Inhibition of the Development of Induced Respiration and Cyanide-insensitive Respiration in Potato Tuber Slices by Cerulenin and Dimethylaminoethanol. Plant Physiol. 1977 Jul;60(1):11–16. doi: 10.1104/pp.60.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Yentur S., Leopold A. C. Respiratory Transition during Seed Germination. Plant Physiol. 1976 Feb;57(2):274–276. doi: 10.1104/pp.57.2.274. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES