Abstract
Total and free amino acid composition of germinating cotton seeds (Gossypium hirsutum L.) was determined. The germinating seeds were separated into cotyledon and developing axis fractions daily and the composition of each tissue was summed to get the whole seed composition. By separating the developing seeds into these two tissue fractions, and determining total and free amino acids, a balance sheet was developed for each amino acid. This technique allowed changes in distribution with time of each amino acid to be followed in each tissue. Data for total content and amount in protein of each amino acid are presented. Asparagine increased in the whole seed, and most of this increase was found in the free pool of the developing axis. Other amino acids (e.g. arginine, glutamic acid) increased in the free pool but showed an over-all decrease, indicating that they were being metabolized. Amino acid contents of storage and nonstorage protein isolates were determined.
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Selected References
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- Atkins C. A., Pate J. S., Sharkey P. J. Asparagine metabolism-key to the nitrogen nutrition of developing legume seeds. Plant Physiol. 1975 Dec;56(6):807–812. doi: 10.1104/pp.56.6.807. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Capdevila A. M., Dure L. Developmental Biochemistry of Cottonseed Embryogenesis and Germination: VIII. Free Amino Acid Pool Composition during Cotyledon Development. Plant Physiol. 1977 Feb;59(2):268–273. doi: 10.1104/pp.59.2.268. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dawson R. Comparison of fractionation of groundnut proteins by two different methods. Anal Biochem. 1971 Jun;41(2):305–313. doi: 10.1016/0003-2697(71)90147-3. [DOI] [PubMed] [Google Scholar]
- King E. E., Lamkin G. E. Isolation of proteins from glanded cottonseed. J Agric Food Chem. 1977 Sep-Oct;25(5):1211–1213. doi: 10.1021/jf60213a039. [DOI] [PubMed] [Google Scholar]
- Schweizer C. J., Ries S. K. Protein content of seed: increase improves growth and yield. Science. 1969 Jul 4;165(3888):73–75. doi: 10.1126/science.165.3888.73. [DOI] [PubMed] [Google Scholar]
- Ting I. P., Zschoche W. C. Asparagine biosynthesis by cotton roots. Carbon dioxide fixation and cyanide incorporation. Plant Physiol. 1970 Apr;45(4):429–434. doi: 10.1104/pp.45.4.429. [DOI] [PMC free article] [PubMed] [Google Scholar]