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. 1985 Apr;19(2):73–78. doi: 10.1007/BF00199712

Antitumor effect induced by a hot water extract of Chlorella vulgaris (CE): Resistance to meth-A tumor growth mediated by CE-induced polymorphonuclear leukocytes

Fumiko Konishi 1,✉, Kuniaki Tanaka 1, Kunisuke Himeno 1, Kazuto Taniguchi 1, Kikuo Nomoto 1
PMCID: PMC11039246  PMID: 3845850

Abstract

When a hot water extract of Chlorella vulgaris (CE) was injected into the peritoneal cavity of BALB/c mice inoculated with syngeneic Meth-A tumor cells, the survival times were strikingly prolonged. Furthermore, peritoneal exudate cells (PEC) rich in polymorphonuclear cells (PMN) obtained from normal mice 24 h after CE injection exhibited an antitumor effect in a Winn-type assay using normal recipients. Such an activity of PEC remained almost intact after T cell or macrophage depletion. However, such PEC did not express an antitumor effect in a Winn-type assay using irradiated recipients. It was suggested that CE-induced PEC, presumably PMN, expressed an antitumor effect in cooperation with a host- or recipient-derived element(s) sensitive to irradiation. The anti-tumor mechanism of CE may be different from that of OK-432, one of the biological response modifiers.

Keywords: Tumor Cell, Tumor Growth, Survival Time, Peritoneal Cavity, Antitumor Effect

References

  • 1.Catalona WJ, Ratliff TL, McCool RE, Heston WDW. Role of antibody in cytotoxicity by lymphocytes armed against 253J bladder cancer line. Int Arch Allergy Appl Immunol. 1981;66:259. doi: 10.1159/000232829. [DOI] [PubMed] [Google Scholar]
  • 2.Chapes SK, Haskill S. Evidence for granulocyte-mediated macrophage activation after C. parvum immunization. Cell Immunol. 1983;75:367. doi: 10.1016/0008-8749(83)90334-9. [DOI] [PubMed] [Google Scholar]
  • 3.Chapes SK, Haskill S. Syngeneic effect between neutrophils and Corynebacterium parvum in the process of macrophage activation. Cancer Res. 1984;44:31. [PubMed] [Google Scholar]
  • 4.Davies M, Sabbadini E. Mechanisms of BCG action: I. The induction of nonspecific helper cells during the potentiation of alloimmune cell-mediated cytotoxic responses. Cancer Immunol Immunother. 1982;14:46. doi: 10.1007/BF00199432. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 5.Dvorak AM, Connell AB, Proppe K, Dvorak HF. Immunologic rejection of mammary adenocarcinoma (TA3-St) in C57BL/6 mice: Participation of neutrophils and activated macrophages with fibrin formation. J Immunol. 1978;120:1240. [PubMed] [Google Scholar]
  • 6.Fisher S, Saffer EA. Tumor cell cytotoxicity by granulocytes from peripheral blood of tumor-bearing mice. J Natl Cancer Inst. 1978;60:687. doi: 10.1093/jnci/60.3.687. [DOI] [PubMed] [Google Scholar]
  • 7.Ghaffar A, Cullen RT, Dunbar N, Woodruff MFA. Antitumor effect in vitro of lymphocytes and macrophages from mice treated with Corynebacterium parvum . Br J Cancer. 1974;29:199. doi: 10.1038/bjc.1974.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 8.Godleski JJ, Lee RE, Leighton J. Studies on the role of polymorphonuclear leukocytes in neoplastic disease with the chick embryo and Walker carcinosarcoma 256 in vivo and in vitro. Cancer Res. 1970;30:1986. [PubMed] [Google Scholar]
  • 9.Gresser I, Maury C, Broaty-Boye D. Mechanism of the antitumor effect of interferon in mice. Nature. 1972;239:167. doi: 10.1038/239167a0. [DOI] [PubMed] [Google Scholar]
  • 10.Harris PJ. Neutrophil product with lymphocyte activating factor activity. Clin Exp Immunol. 1982;50:474. [PMC free article] [PubMed] [Google Scholar]
  • 11.Hibbs JB, Lambert L, Remington J. Possible role of the macrophage-mediated nonspecific cytotoxicity in tumor resistance. Nature New Biol. 1972;235:48. doi: 10.1038/newbio235048a0. [DOI] [PubMed] [Google Scholar]
  • 12.Jacobsen F. Complement-dependent in vitro cytotoxicity against autologous invasive bladder tumor cells in human. Acta Pathol Microbiol Scan. 1981;89:297. doi: 10.1111/j.1699-0463.1981.tb02703.x. [DOI] [PubMed] [Google Scholar]
  • 13.Katano M, Torisu M. Neutrophil-mediated tumor cell destruction in cancer ascites. Am Cancer Soc. 1982;50:62. doi: 10.1002/1097-0142(19820701)50:1<62::aid-cncr2820500113>3.0.co;2-0. [DOI] [PubMed] [Google Scholar]
  • 14.Kirchner H, Muchmore AV, Chused TM, Holden HT, Herberman RB. Inhibiton of proliferation of lymphoma cells and T lymphocytes by suppressor cells from spleens of tumor-bearing mice. J Immunol. 1975;114:206. [PubMed] [Google Scholar]
  • 15.Kojima M, Kasajima T, Imai Y, Kobayashi S, Dobashi M, Uemura T. A new Chlorella polysaccharide and its accelerating effect on the phagocytic activity of the reticuloendothelial system. Recent Adv RES Res. 1973;13:101. [Google Scholar]
  • 16.Korec S, Herberman RB, Dean JH, Cannon GB. Cytostasis of tumor cell lines by human granulocytes. Cell Immunol. 1980;53:104. doi: 10.1016/0008-8749(80)90430-x. [DOI] [PubMed] [Google Scholar]
  • 17.Lake W, Bice D, Schwartz HJ, Salvaggio J. Suppression of in vitro antigen-induced lymphocyte transformation by carrageen-an, a macrophage toxic antigen. J Immunol. 1971;107:1745. [PubMed] [Google Scholar]
  • 18.Miller JF, Brunner KT, Sprent J, Russell PJ, Mitchell GF. Thymus-derived cells as killer cells in cell-mediated immunity. Tansplant Proc. 1971;3:915. [PubMed] [Google Scholar]
  • 19.Murayama T, Natsuume-Sakai S, Ryoyama K, Koshimura S. Studies of the properties of streptococcal preparation, OK-432(NSC-B116209), as an immunopotentiator: II. Mechanism of macrophage activation by OK-432. Cancer Immunol Immunother. 1982;12:141. [Google Scholar]
  • 20.Okamoto K, Suzuki T, Murakami T, Miyata H. A further study on dietary effects on blood pressure and incidence of stroke in SHRSP. Jpn Heart J. 1979;20:334. [Google Scholar]
  • 21.Pickaver AH, Ratcliff NA, Williams AE, Smith H. Cytotoxic effects of peritoneal neutrophils on a syngeneic rat tumor. Nature New Biol. 1972;235:186. doi: 10.1038/newbio235186a0. [DOI] [PubMed] [Google Scholar]
  • 22.Sano T, Tanaka U, Watanabe T, Tanaka K, Okuda M. The effect of Chlorella on dietary hypercholesteremia of rats. J Jpn Atheroscler Soc. 1978;6:195. [Google Scholar]
  • 23.Tanaka K, Konishi F, Himeno K, Taniguchi K, Nomoto K. Augmentation of antitumor resistance by a strain of unicellular green-algae: Chlorella vulgaris . Cancer Immunol Immunother. 1984;17:90. doi: 10.1007/BF00200042. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 24.Vischer TL, Bretz U, Baggiolini M. In vitro stimulation of lymphocytes by neutral proteinases from human polymorphonuclear leukocyte granules. J Exp Med. 1976;144:863. doi: 10.1084/jem.144.4.863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 25.Winn HJ. Immune mechanisms in homotransplantation: II. Quantitative assay of the immunologic activity of lymphoid cells stimulated by tumor homografts. J Immunol. 1961;86:228. [PubMed] [Google Scholar]
  • 26.Wright SC, Bonavida B. Studies on the mechanism of natural killer cell-mediated cytotoxicity: II. Activation of NK cells by interferon augments the lytic activity of released natural killer cytotoxic factors (NKCF) J Immunol. 1983;130:2965. [PubMed] [Google Scholar]
  • 27.Yoshinaga M, Nishime K, Nakamura S, Goto F. A PMN-derived factor that enhances DNA-synthesis in PHA or antigen-stimulated lymphocytes. J Immunol. 1980;124:94. [PubMed] [Google Scholar]

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