Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1991 Dec 1;280(Pt 2):515–519. doi: 10.1042/bj2800515

Inhibition by pertussis toxin of the activation of Na(+)-dependent uridine transport in dimethyl-sulphoxide-induced HL-60 leukaemia cells.

J A Sokoloski 1, A C Sartorelli 1, R E Handschumacher 1, C W Lee 1
PMCID: PMC1130578  PMID: 1747127

Abstract

The effects of pertussis toxin on the Na(+)-dependent transport of uridine were studied in HL-60 leukaemia cells induced to differentiate along the granulocytic or monocytic pathways by dimethyl sulphoxide (DMSO) or phorbol 12-myristate 13-acetate (PMA) respectively. Pertussis toxin at 50 ng/ml completely inhibited the activation of Na(+)-dependent uridine transport and consequently prevented the formation of intracellular pools of free uridine which occurs in HL-60 cells induced to differentiate by DMSO. The inhibition of Na(+)-dependent uridine transport by pertussis toxin in cells exposed to DMSO was associated with a 14-fold decrease in affinity, with no change in Vmax. Pertussis toxin, however, had no effect on Na(+)-dependent uridine transport in PMA-induced HL-60 cells. Furthermore, 500 ng of cholera toxin/ml had no effect on the Na(+)-dependent uptake of uridine in DMSO-treated HL-60 cells. These results suggest that the activation of the Na(+)-dependent transport of uridine in HL-60 cells induced to differentiate along the granulocytic pathway by DMSO is coupled to a pertussis-toxin-sensitive guanine-nucleotide binding protein (G-protein).

Full text

PDF
518

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barrowman M. M., Cockcroft S., Gomperts B. D. Two roles for guanine nucleotides in the stimulus-secretion sequence of neutrophils. Nature. 1986 Feb 6;319(6053):504–507. doi: 10.1038/319504a0. [DOI] [PubMed] [Google Scholar]
  2. Belt J. A., Noel L. D. Isolation and characterization of a mutant of L1210 murine leukemia deficient in nitrobenzylthioinosine-insensitive nucleoside transport. J Biol Chem. 1988 Sep 25;263(27):13819–13822. [PubMed] [Google Scholar]
  3. Burch R. M., Luini A., Axelrod J. Phospholipase A2 and phospholipase C are activated by distinct GTP-binding proteins in response to alpha 1-adrenergic stimulation in FRTL5 thyroid cells. Proc Natl Acad Sci U S A. 1986 Oct;83(19):7201–7205. doi: 10.1073/pnas.83.19.7201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burgoyne R. D. G proteins: control of exocytosis. Nature. 1987 Jul 9;328(6126):112–113. doi: 10.1038/328112a0. [DOI] [PubMed] [Google Scholar]
  5. Cantiello H. F., Patenaude C. R., Ausiello D. A. G protein subunit, alpha i-3, activates a pertussis toxin-sensitive Na+ channel from the epithelial cell line, A6. J Biol Chem. 1989 Dec 15;264(35):20867–20870. [PubMed] [Google Scholar]
  6. Cass C. E., Paterson A. R. Mediated transport of nucleosides by human erythrocytes. Specificity toward purine nucleosides as permeants. Biochim Biophys Acta. 1973 Feb 16;291(3):734–746. doi: 10.1016/0005-2736(73)90477-x. [DOI] [PubMed] [Google Scholar]
  7. Cass C. E., Paterson A. R. Mediated transport of nucleosides in human erythrocytes. Accelerative exchange diffusion of uridine and thymidine and specificity toward pyrimidine nucleosides as permeants. J Biol Chem. 1972 May 25;247(10):3314–3320. [PubMed] [Google Scholar]
  8. Codina J., Yatani A., Grenet D., Brown A. M., Birnbaumer L. The alpha subunit of the GTP binding protein Gk opens atrial potassium channels. Science. 1987 Apr 24;236(4800):442–445. doi: 10.1126/science.2436299. [DOI] [PubMed] [Google Scholar]
  9. Darnowski J. W., Handschumacher R. E. Tissue uridine pools: evidence in vivo of a concentrative mechanism for uridine uptake. Cancer Res. 1986 Jul;46(7):3490–3494. [PubMed] [Google Scholar]
  10. Darnowski J. W., Holdridge C., Handschumacher R. E. Concentrative uridine transport by murine splenocytes: kinetics, substrate specificity, and sodium dependency. Cancer Res. 1987 May 15;47(10):2614–2619. [PubMed] [Google Scholar]
  11. Gierschik P., Sidiropoulos D., Spiegel A., Jakobs K. H. Purification and immunochemical characterization of the major pertussis-toxin-sensitive guanine-nucleotide-binding protein of bovine-neutrophil membranes. Eur J Biochem. 1987 May 15;165(1):185–194. doi: 10.1111/j.1432-1033.1987.tb11210.x. [DOI] [PubMed] [Google Scholar]
  12. Gilman A. G. G proteins and dual control of adenylate cyclase. Cell. 1984 Mar;36(3):577–579. doi: 10.1016/0092-8674(84)90336-2. [DOI] [PubMed] [Google Scholar]
  13. Gilman A. G. G proteins: transducers of receptor-generated signals. Annu Rev Biochem. 1987;56:615–649. doi: 10.1146/annurev.bi.56.070187.003151. [DOI] [PubMed] [Google Scholar]
  14. Gomperts B. D., Cockcroft S., Howell T. W., Nüsse O., Tatham P. E. The dual effector system for exocytosis in mast cells: obligatory requirement for both Ca2+ and GTP. Biosci Rep. 1987 May;7(5):369–381. doi: 10.1007/BF01362501. [DOI] [PubMed] [Google Scholar]
  15. Holz G. G., 4th, Rane S. G., Dunlap K. GTP-binding proteins mediate transmitter inhibition of voltage-dependent calcium channels. Nature. 1986 Feb 20;319(6055):670–672. doi: 10.1038/319670a0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Jarvis S. M. Characterization of sodium-dependent nucleoside transport in rabbit intestinal brush-border membrane vesicles. Biochim Biophys Acta. 1989 Feb 13;979(1):132–138. doi: 10.1016/0005-2736(89)90533-6. [DOI] [PubMed] [Google Scholar]
  17. Jarvis S. M., Young J. D. Nucleoside translocation in sheep reticulocytes and fetal erythrocytes: a proposed model for the nucleoside transporter. J Physiol. 1982 Mar;324:47–66. doi: 10.1113/jphysiol.1982.sp014100. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Jarvis S. M., Young J. D. Nucleoside transport in rat erythrocytes: two components with differences in sensitivity to inhibition by nitrobenzylthioinosine and p-chloromercuriphenyl sulfonate. J Membr Biol. 1986;93(1):1–10. doi: 10.1007/BF01871013. [DOI] [PubMed] [Google Scholar]
  19. Katada T., Bokoch G. M., Northup J. K., Ui M., Gilman A. G. The inhibitory guanine nucleotide-binding regulatory component of adenylate cyclase. Properties and function of the purified protein. J Biol Chem. 1984 Mar 25;259(6):3568–3577. [PubMed] [Google Scholar]
  20. Le Hir M., Dubach U. C. Uphill transport of pyrimidine nucleosides in renal brush border vesicles. Pflugers Arch. 1985 Jul;404(3):238–243. doi: 10.1007/BF00581245. [DOI] [PubMed] [Google Scholar]
  21. Lee C. W., Cheeseman C. I., Jarvis S. M. Na+- and K+-dependent uridine transport in rat renal brush-border membrane vesicles. Biochim Biophys Acta. 1988 Jul 7;942(1):139–149. doi: 10.1016/0005-2736(88)90283-0. [DOI] [PubMed] [Google Scholar]
  22. Lee C. W., Cheeseman C. I., Jarvis S. M. Transport characteristics of renal brush border Na(+)- and K(+)-dependent uridine carriers. Am J Physiol. 1990 May;258(5 Pt 2):F1203–F1210. doi: 10.1152/ajprenal.1990.258.5.F1203. [DOI] [PubMed] [Google Scholar]
  23. Lee C. W., Sokoloski J. A., Sartorelli A. C., Handschumacher R. E. Induction of the differentiation of HL-60 cells by phorbol 12-myristate 13-acetate activates a Na(+)-dependent uridine-transport system. Involvement of protein kinase C. Biochem J. 1991 Feb 15;274(Pt 1):85–90. doi: 10.1042/bj2740085. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Moss J., Vaughan M. Mechanism of action of choleragen. Evidence for ADP-ribosyltransferase activity with arginine as an acceptor. J Biol Chem. 1977 Apr 10;252(7):2455–2457. [PubMed] [Google Scholar]
  25. Nasmith P. E., Mills G. B., Grinstein S. Guanine nucleotides induce tyrosine phosphorylation and activation of the respiratory burst in neutrophils. Biochem J. 1989 Feb 1;257(3):893–897. doi: 10.1042/bj2570893. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Nishizuka Y. The role of protein kinase C in cell surface signal transduction and tumour promotion. Nature. 1984 Apr 19;308(5961):693–698. doi: 10.1038/308693a0. [DOI] [PubMed] [Google Scholar]
  27. Plagemann P. G., Wohlhueter R. M. Nucleoside transport in cultured mammalian cells. Multiple forms with different sensitivity to inhibition by nitrobenzylthioinosine or hypoxanthine. Biochim Biophys Acta. 1984 Jun 13;773(1):39–52. doi: 10.1016/0005-2736(84)90548-0. [DOI] [PubMed] [Google Scholar]
  28. Schwenk M., Hegazy E., Lopez del Pino V. Uridine uptake by isolated intestinal epithelial cells of guinea pig. Biochim Biophys Acta. 1984 Dec 11;805(4):370–374. doi: 10.1016/0167-4889(84)90020-x. [DOI] [PubMed] [Google Scholar]
  29. Smith C. D., Cox C. C., Snyderman R. Receptor-coupled activation of phosphoinositide-specific phospholipase C by an N protein. Science. 1986 Apr 4;232(4746):97–100. doi: 10.1126/science.3006254. [DOI] [PubMed] [Google Scholar]
  30. Sokoloski J. A., Blair O. C., Sartorelli A. C. Alterations in glycoprotein synthesis and guanosine triphosphate levels associated with the differentiation of HL-60 leukemia cells produced by inhibitors of inosine 5'-phosphate dehydrogenase. Cancer Res. 1986 May;46(5):2314–2319. [PubMed] [Google Scholar]
  31. Spiegel A. M., Brown E. M., Fedak S. A., Woodard C. J., Aurbach G. D. Holocatalytic state of adenylate cyclase in turkey erythrocyte membranes: formation with guanylylimidodiphosphate plus isoproterenol without effect on affinity of beta-receptor. J Cyclic Nucleotide Res. 1976;2(1):47–56. [PubMed] [Google Scholar]
  32. Trimble M. E., Coulson R. Adenosine transport in perfused rat kidney and renal cortical membrane vesicles. Am J Physiol. 1984 Jun;246(6 Pt 2):F794–F803. doi: 10.1152/ajprenal.1984.246.6.F794. [DOI] [PubMed] [Google Scholar]
  33. West R. E., Jr, Moss J., Vaughan M., Liu T., Liu T. Y. Pertussis toxin-catalyzed ADP-ribosylation of transducin. Cysteine 347 is the ADP-ribose acceptor site. J Biol Chem. 1985 Nov 25;260(27):14428–14430. [PubMed] [Google Scholar]
  34. Williams T. C., Jarvis S. M. Multiple sodium-dependent nucleoside transport systems in bovine renal brush-border membrane vesicles. Biochem J. 1991 Feb 15;274(Pt 1):27–33. doi: 10.1042/bj2740027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Young J. D., Jarvis S. M. Nucleoside transport in animal cells. Biosci Rep. 1983 Apr;3(4):309–322. doi: 10.1007/BF01122895. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES