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. 1992 Apr 1;283(Pt 1):99–104. doi: 10.1042/bj2830099

Identification of the reaction products of the purified hyaluronidase from stonefish (Synanceja horrida) venom.

K Sugahara 1, S Yamada 1, M Sugiura 1, K Takeda 1, R Yuen 1, H E Khoo 1, C H Poh 1
PMCID: PMC1130999  PMID: 1567384

Abstract

Recently we purified to homogeneity hyaluronidase from stonefish (Synanceja horrida) venom, for the first time from a marine source [Poh, Yuen, Chung & Khoo (1992) Comp. Biochem. Physiol., in the press]. In the present study the reaction products of the hyaluronidase purified from stonefish venom were analysed. It produced tetra-, hexa-, octa- and deca-saccharides as major end products, but not disaccharides. The structure of the tetrasaccharide product was determined by enzymic analysis, in conjunction with h.p.l.c. and by 1H n.m.r., as GlcA beta 1-3GlcNAc beta 1-4GlcA beta 1-3GlcNAc. Chemical shifts of the structural-reporter-group protons of the constituent monosaccharides for the tetrasaccharide have been assigned. The enzyme did not act on chondroitin sulphate or dermatan sulphate. The results indicate that the stonefish hyaluronidase is an endo-beta-N-acetylglucosaminidase specific for hyaluronate.

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Selected References

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  1. BITTER T., MUIR H. M. A modified uronic acid carbazole reaction. Anal Biochem. 1962 Oct;4:330–334. doi: 10.1016/0003-2697(62)90095-7. [DOI] [PubMed] [Google Scholar]
  2. Borders C. L., Jr, Raftery M. A. Purification and partial characterization of testicular hyaluronidase. J Biol Chem. 1968 Jul 10;243(13):3756–3762. [PubMed] [Google Scholar]
  3. DI FERRANTE N. Turbidimetric measurement of acid mucopolysaccharides and hyaluronidase activity. J Biol Chem. 1956 May;220(1):303–306. [PubMed] [Google Scholar]
  4. Gold E. W. Purification and properties of hyaluronidase from human liver. Differences from and similarities to the testicular enzyme. Biochem J. 1982 Jul 1;205(1):69–74. doi: 10.1042/bj2050069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Joy M. B., Dodgson K. S., Olavesen A. H., Gacesa P. The purification and some properties of pig liver hyaluronidase. Biochim Biophys Acta. 1985 Feb 15;838(2):257–263. doi: 10.1016/0304-4165(85)90087-x. [DOI] [PubMed] [Google Scholar]
  6. Kresse H., Glössl J. Glycosaminoglycan degradation. Adv Enzymol Relat Areas Mol Biol. 1987;60:217–311. doi: 10.1002/9780470123065.ch4. [DOI] [PubMed] [Google Scholar]
  7. LINKER A., MEYER K., HOFFMAN P. The production of hyaluronate oligosaccharides by leech hyaluronidase and alkali. J Biol Chem. 1960 Apr;235:924–927. [PubMed] [Google Scholar]
  8. MEYER K., RAPPORT M. M. Hyaluronidases. Adv Enzymol Relat Subj Biochem. 1952;13:199–236. doi: 10.1002/9780470122587.ch6. [DOI] [PubMed] [Google Scholar]
  9. Maclean D., Fishbein M. C., Maroko P. R., Braunwald E. Hyaluronidase-induced reductions in myocardial infarct size. Science. 1976 Oct 8;194(4261):199–200. doi: 10.1126/science.959848. [DOI] [PubMed] [Google Scholar]
  10. Ohya T., Kaneko Y. Novel hyaluronidase from streptomyces. Biochim Biophys Acta. 1970 Mar 18;198(3):607–609. doi: 10.1016/0005-2744(70)90139-7. [DOI] [PubMed] [Google Scholar]
  11. Owen M. D. The venom system and venom hyaluronidase of the African honeybee (Apis mellifera adansonii). Toxicon. 1983;21(1):171–174. doi: 10.1016/0041-0101(83)90061-2. [DOI] [PubMed] [Google Scholar]
  12. Pukrittayakamee S., Warrell D. A., Desakorn V., McMichael A. J., White N. J., Bunnag D. The hyaluronidase activities of some Southeast Asian snake venoms. Toxicon. 1988;26(7):629–637. doi: 10.1016/0041-0101(88)90245-0. [DOI] [PubMed] [Google Scholar]
  13. Ramanaiah M., Parthasarathy P. R., Venkaiah B. Isolation and characterization of hyaluronidase from scorpion (Heterometrus fulvipes) venom. Biochem Int. 1990;20(2):301–310. [PubMed] [Google Scholar]
  14. SILBERT J. E., NAGAI Y., GROSS J. HYALURONIDASE FROM TADPOLE TISSUE. J Biol Chem. 1965 Apr;240:1509–1511. [PubMed] [Google Scholar]
  15. Sugahara K., Masuda M., Harada T., Yamashina I., de Waard P., Vliegenthart J. F. Structural studies on sulfated oligosaccharides derived from the carbohydrate-protein linkage region of chondroitin sulfate proteoglycans of whale cartilage. Eur J Biochem. 1991 Dec 18;202(3):805–811. doi: 10.1111/j.1432-1033.1991.tb16436.x. [DOI] [PubMed] [Google Scholar]
  16. Tan Y. H., Bowness J. M. Canine submandibular-gland hyaluronidase. Identification and subcellular distribution. Biochem J. 1968 Nov;110(1):9–17. doi: 10.1042/bj1100009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tan Y. H., Bowness J. M. Canine submandibular-gland hyaluronidase. Purification and properties. Biochem J. 1968 Nov;110(1):19–25. doi: 10.1042/bj1100019. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tu A. T., Hendon R. R. Characterization of lizard venom hyaluronidase and evidence for its action as a spreading factor. Comp Biochem Physiol B. 1983;76(2):377–383. doi: 10.1016/0305-0491(83)90086-x. [DOI] [PubMed] [Google Scholar]
  19. Ulrich H. P., Klein U., von Figura K. Degradation of even-numbered reduced and non-reduced hyaluronate oligosaccharides with D-glucuronic acid or N-acetyl-D-glucosamine as non-reducing terminal by chondroitin ABC and AC lyases. Hoppe Seylers Z Physiol Chem. 1979 Oct;360(10):1457–1463. doi: 10.1515/bchm2.1979.360.2.1457. [DOI] [PubMed] [Google Scholar]
  20. Vikha I. V., Sakharovsky V. G., Bystrov V. F., Khorlin A. Y. A proton magnetic resonance kinetic approach to the stereochemistry of carbohydrate enzymic hydrolysis. Hydrolysis of hyaluronic acid by testicular hyaluronidase. Carbohydr Res. 1972 Nov;25(1):143–152. doi: 10.1016/s0008-6215(00)82754-8. [DOI] [PubMed] [Google Scholar]
  21. Xu X., Wang X. S., Xi X. T., Liu J., Huang J. T., Lu Z. X. Purification and partial characterization of hyaluronidase from five pace snake (Agkistrodon acutus) venom. Toxicon. 1982;20(6):973–981. doi: 10.1016/0041-0101(82)90099-x. [DOI] [PubMed] [Google Scholar]
  22. Yamada M., Hasegawa E., Kanamori M. Purification of hyaluronidase from human placenta. J Biochem. 1977 Feb;81(2):485–494. doi: 10.1093/oxfordjournals.jbchem.a131482. [DOI] [PubMed] [Google Scholar]

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