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. 1992 Apr 15;283(Pt 2):379–383. doi: 10.1042/bj2830379

Spiking in cytosolic calcium concentration in single fibrinogen-bound fura-2-loaded human platelets.

J W Heemskerk 1, J Hoyland 1, W T Mason 1, S O Sage 1
PMCID: PMC1131044  PMID: 1575681

Abstract

Fura-2-loaded human platelets were immobilized on a fibrinogen-coated surface and the cytosolic free calcium concentration ([Ca2+]i) was measured in single platelets by low-light-level video-ratio image-processing of the optical probe signal. Some fibrinogen-bound platelets showed repetitive spiking in [Ca2+]i with a mean frequency of about 2/min, which increased to 5/min in the presence of ADP. Other cells showed no activity until the addition of agonist. When immobilized in the presence of prostaglandin I2 and the fibrinogen antagonist Arg-Gly-Asp-Ser, the platelets adhered less firmly to fibrinogen, and in many [Ca2+]i remained low and constant. Subsequent activation of such platelets with ADP evoked oscillations in [Ca2+]i with a peak frequency of about 5/min and which persisted for at least 5 min. These results indicate that human platelets, like many other non-excitable cells, have an elaborate system of calcium signalling involving spiking.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akerman S. N., Zorec R., Cheek T. R., Moreton R. B., Berridge M. J., Mason W. T. Fura-2 imaging of thyrotropin-releasing hormone and dopamine effects on calcium homeostasis of bovine lactotrophs. Endocrinology. 1991 Jul;129(1):475–488. doi: 10.1210/endo-129-1-475. [DOI] [PubMed] [Google Scholar]
  2. Berridge M. J. Calcium oscillations. J Biol Chem. 1990 Jun 15;265(17):9583–9586. [PubMed] [Google Scholar]
  3. Berridge M. J., Irvine R. F. Inositol phosphates and cell signalling. Nature. 1989 Sep 21;341(6239):197–205. doi: 10.1038/341197a0. [DOI] [PubMed] [Google Scholar]
  4. Billah M. M., Lapetina E. G. Evidence for multiple metabolic pools of phosphatidylinositol in stimulated platelets. J Biol Chem. 1982 Oct 25;257(20):11856–11859. [PubMed] [Google Scholar]
  5. D'Souza S. E., Ginsberg M. H., Matsueda G. R., Plow E. F. A discrete sequence in a platelet integrin is involved in ligand recognition. Nature. 1991 Mar 7;350(6313):66–68. doi: 10.1038/350066a0. [DOI] [PubMed] [Google Scholar]
  6. Daniel J. L., Dangelmaier C. A., Selak M., Smith J. B. ADP stimulates IP3 formation in human platelets. FEBS Lett. 1986 Oct 6;206(2):299–303. doi: 10.1016/0014-5793(86)81000-6. [DOI] [PubMed] [Google Scholar]
  7. Davies T. A., Drotts D. L., Weil G. J., Simons E. R. Cytoplasmic Ca2+ is necessary for thrombin-induced platelet activation. J Biol Chem. 1989 Nov 25;264(33):19600–19606. [PubMed] [Google Scholar]
  8. Fisher G. J., Bakshian S., Baldassare J. J. Activation of human platelets by ADP causes a rapid rise in cytosolic free calcium without hydrolysis of phosphatidylinositol-4,5-bisphosphate. Biochem Biophys Res Commun. 1985 Jun 28;129(3):958–964. doi: 10.1016/0006-291x(85)91984-9. [DOI] [PubMed] [Google Scholar]
  9. Harootunian A. T., Kao J. P., Paranjape S., Tsien R. Y. Generation of calcium oscillations in fibroblasts by positive feedback between calcium and IP3. Science. 1991 Jan 4;251(4989):75–78. doi: 10.1126/science.1986413. [DOI] [PubMed] [Google Scholar]
  10. Heemskerk J. W., Feijge M. A., Rietman E., Hornstra G. Rat platelets are deficient in internal Ca2+ release and require influx of extracellular Ca2+ for activation. FEBS Lett. 1991 Jun 24;284(2):223–226. doi: 10.1016/0014-5793(91)80690-5. [DOI] [PubMed] [Google Scholar]
  11. Jacob R., Merritt J. E., Hallam T. J., Rink T. J. Repetitive spikes in cytoplasmic calcium evoked by histamine in human endothelial cells. Nature. 1988 Sep 1;335(6185):40–45. doi: 10.1038/335040a0. [DOI] [PubMed] [Google Scholar]
  12. Jennings L. K., Dockter M. E., Wall C. D., Fox C. F., Kennedy D. M. Calcium mobilization in human platelets using indo-1 and flow cytometry. Blood. 1989 Dec;74(8):2674–2680. [PubMed] [Google Scholar]
  13. Loessberg P. A., Zhao H., Muallem S. Synchronized oscillation of Ca2+ entry and Ca2+ release in agonist-stimulated AR42J cells. J Biol Chem. 1991 Jan 25;266(3):1363–1366. [PubMed] [Google Scholar]
  14. Neylon C. B., Hoyland J., Mason W. T., Irvine R. F. Spatial dynamics of intracellular calcium in agonist-stimulated vascular smooth muscle cells. Am J Physiol. 1990 Oct;259(4 Pt 1):C675–C686. doi: 10.1152/ajpcell.1990.259.4.C675. [DOI] [PubMed] [Google Scholar]
  15. Nishio H., Ikegami Y., Segawa T. Fluorescence digital image analysis of serotonin-induced calcium oscillations in single blood platelets. Cell Calcium. 1991 Feb-Mar;12(2-3):177–184. doi: 10.1016/0143-4160(91)90019-b. [DOI] [PubMed] [Google Scholar]
  16. Petersen O. H., Gallacher D. V., Wakui M., Yule D. I., Petersen C. C., Toescu E. C. Receptor-activated cytoplasmic Ca2+ oscillations in pancreatic acinar cells: generation and spreading of Ca2+ signals. Cell Calcium. 1991 Feb-Mar;12(2-3):135–144. doi: 10.1016/0143-4160(91)90015-7. [DOI] [PubMed] [Google Scholar]
  17. Plow E. F., Pierschbacher M. D., Ruoslahti E., Marguerie G. A., Ginsberg M. H. The effect of Arg-Gly-Asp-containing peptides on fibrinogen and von Willebrand factor binding to platelets. Proc Natl Acad Sci U S A. 1985 Dec;82(23):8057–8061. doi: 10.1073/pnas.82.23.8057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rink T. J., Hallam T. J. Calcium signalling in non-excitable cells: notes on oscillations and store refilling. Cell Calcium. 1989 Jul;10(5):385–395. doi: 10.1016/0143-4160(89)90064-x. [DOI] [PubMed] [Google Scholar]
  19. Rink T. J., Sage S. O. Calcium signaling in human platelets. Annu Rev Physiol. 1990;52:431–449. doi: 10.1146/annurev.ph.52.030190.002243. [DOI] [PubMed] [Google Scholar]
  20. Sage S. O., Rink T. J. The kinetics of changes in intracellular calcium concentration in fura-2-loaded human platelets. J Biol Chem. 1987 Dec 5;262(34):16364–16369. [PubMed] [Google Scholar]
  21. Siess W. Molecular mechanisms of platelet activation. Physiol Rev. 1989 Jan;69(1):58–178. doi: 10.1152/physrev.1989.69.1.58. [DOI] [PubMed] [Google Scholar]
  22. Tsunoda Y., Matsuno K., Tashiro Y. Spatial distribution and temporal change of cytoplasmic free calcium in human platelets. Biochem Biophys Res Commun. 1988 Nov 15;156(3):1152–1159. doi: 10.1016/s0006-291x(88)80753-8. [DOI] [PubMed] [Google Scholar]
  23. Watson S. P., McConnell R. T., Lapetina E. G. The rapid formation of inositol phosphates in human platelets by thrombin is inhibited by prostacyclin. J Biol Chem. 1984 Nov 10;259(21):13199–13203. [PubMed] [Google Scholar]
  24. van Willigen G., Akkerman J. W. Protein kinase C and cyclic AMP regulate reversible exposure of binding sites for fibrinogen on the glycoprotein IIB-IIIA complex of human platelets. Biochem J. 1991 Jan 1;273(Pt 1):115–120. doi: 10.1042/bj2730115. [DOI] [PMC free article] [PubMed] [Google Scholar]

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